MLC-specific suppressor T lymphocytes in man. I. Their induction in vitro by soluble HLA-DR antigens

R. S. Smeraldi, G. Fabio, P. Bonara, M. G. Villa, M. Vanoli, C. Pettenati, C. Rugarli, C. Zanussi

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Human T lymphocytes precultured for 36 hr in the presence of soluble HLA-DR antigens suppress the MLR response of autologous peripheral blood lymphocytes to allogeneic stimulating cells. The suppression is DR antigen-specific in that it appears that the MLR stimulating cell donor and the soluble suppressor-inducing antigen must share DR specificities. The soluble DR antigens were fractionated from the sera of normal donors using QAE-Sephadex chromatography and CNBr-activated Sepharose immunoadsorption. Similarly prepared HLA-A and -B antigens failed to induce suppressive activity. The suppressive activity of DR-antigen cultured T cells is resistant to mitomycin C treatment and, further, the antigen specificity is maintained with or without mitomycin C treatment. The kinetics of suppressor cell induction as well as the kinetics of suppression in the test MLR cultures are presented. The implications of these results are discussed.

Original languageEnglish
Pages (from-to)1394-1398
Number of pages5
JournalJournal of Immunology
Issue number3
Publication statusPublished - 1982

ASJC Scopus subject areas

  • Immunology


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