Mlc1p promotes septum closure during cytokinesis via the IQ motifs of the vesicle motor Myo2p

Wolfgang Wagner, Pamela Bielli, Stefan Wacha, Antonella Ragnini-Wilson

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Little is known about the molecular machinery that directs secretory vesicles to the site of cell separation during cytokinesis. We show that in Saccharomyces cerevisiae, the class V myosin Myo2p and the Rab/Ypt Sec4p, that are required for vesicle polarization processes at all stages of the cell cycle, form a complex with each other and with a myosin light chain, Mlc1p, that is required for actomyosin ring assembly and cytokinesis. Mlc1p travels on secretory vesicles and forms a complex(es) with Myo2p and/or Sec4p. Its functional interaction with Myo2p is essential during cytokinesis to target secretory vesicles to fill the mother bud neck. The role of Mlc1p in actomyosin ring assembly instead is dispensable for this process. Therefore, in yeast, as recently shown in mammals, class V myosins associate with vesicles via the formation of a complex with Rab/Ypt proteins. Furthermore, myosin light chains, via their ability to be transported by secretory vesicles and to interact with class V myosin IQ motifs, can regulate vesicle polarization processes at a specific location and stage of the cell cycle.

Original languageEnglish
Pages (from-to)6397-6408
Number of pages12
JournalEMBO Journal
Volume21
Issue number23
DOIs
Publication statusPublished - Dec 2 2002

Fingerprint

Myosin Type V
Cytokinesis
Secretory Vesicles
Actomyosin
Myosin Light Chains
Yeast
Cells
Polarization
Cell Cycle
Mammals
Machinery
Cell Separation
Saccharomyces cerevisiae
Yeasts
Proteins

Keywords

  • Class V myosin
  • Cytokinesis
  • Myosin light chain
  • Polarized vesicle transport
  • Rab
  • Ypt proteins

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Mlc1p promotes septum closure during cytokinesis via the IQ motifs of the vesicle motor Myo2p. / Wagner, Wolfgang; Bielli, Pamela; Wacha, Stefan; Ragnini-Wilson, Antonella.

In: EMBO Journal, Vol. 21, No. 23, 02.12.2002, p. 6397-6408.

Research output: Contribution to journalArticle

Wagner, Wolfgang ; Bielli, Pamela ; Wacha, Stefan ; Ragnini-Wilson, Antonella. / Mlc1p promotes septum closure during cytokinesis via the IQ motifs of the vesicle motor Myo2p. In: EMBO Journal. 2002 ; Vol. 21, No. 23. pp. 6397-6408.
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