MMP-7 and fcDNA serum levels in early NSCLC and idiopathic interstitial pneumonia: Preliminary study

Paola Ulivi, Gian Luca Casoni, Giovanni Foschi, Emanuela Scarpi, Sara Tomassetti, Micaela Romagnoli, Claudia Ravaglia, Marta Mengozzi, Wainer Zoli, Venerino Poletti

Research output: Contribution to journalArticlepeer-review

Abstract

A non-invasive test to facilitate the diagnosis of non-small cell lung cancer (NSCLC) and idiopathic pulmonary fibrosis (IPF) is still not available and represents an important goal. Forty-eight patients with stage I NSCLC, 45 with IPF, 30 with other idiopathic interstitial pneumonias (IIPs) including idiopathic non-specific interstitial pneumonia (NSIP) and chronic hypersensitivity pneumonitis (HP), 35 with diffuse non-malignant disease and 30 healthy donors were enrolled onto the study. Free circulating (fc)DNA and MMP-7 levels were evaluated by Real Time PCR and ELISA, respectively. Median fcDNA levels were similar in NSCLC (127 ng/mL, range 23.6-345 ng/mL) and IPF (106 ng/mL, range 22-224 ng/mL) patients, and significantly lower in IIPs patients, in individuals with other diseases and in healthy donors (p <0.05). Conversely, median MMP-7 values were significantly higher in IPF patients (9.10 ng/mL, range 3.88-19.72 ng/mL) than in those with NSCLC (6.31 ng/mL, range 3.38-16.36 ng/mL; p <0.0001), NSIP (6.50 ng/mL, range 1.50-22.47 ng/mL; p = 0.007), other diseases (5.41 ng/mL, range 1.78-15.91, p <0.0001) or healthy donors (4.35 ng/mL, range 2.45-7.23; p <0.0001). Serum MMP-7 levels seem to be capable of distinguishing IPF patients from those with any other lung disease. fcDNA levels were similar in NSCLC and IPF patients, confirming its potential role as a biomarker, albeit non-specific, for the differential diagnosis of NSCLC.

Original languageEnglish
Pages (from-to)24097-24112
Number of pages16
JournalInternational Journal of Molecular Sciences
Volume14
Issue number12
DOIs
Publication statusPublished - Dec 11 2013

Keywords

  • FcDNA
  • IIPS
  • IPF
  • MMP-7
  • NSCLC
  • NSIP
  • Serum

ASJC Scopus subject areas

  • Computer Science Applications
  • Molecular Biology
  • Catalysis
  • Inorganic Chemistry
  • Spectroscopy
  • Organic Chemistry
  • Physical and Theoretical Chemistry

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