MMP2-9 cleavage of dystroglycan alters the size and molecular composition of Schwann cell domains

Felipe A. Court, Desirée Zambroni, Ernesto Pavoni, Cristina Colombelli, Chiara Baragli, Gianluca Figlia, Lydia Sorokin, William Ching, James L. Salzer, Lawrence Wrabetz, M. Laura Feltri

Research output: Contribution to journalArticlepeer-review

Abstract

Myelinating glial cells exhibit a spectacular cytoarchitecture, because they polarize on multiple axes and domains. How this occurs is essentially unknown. The dystroglycan-dystrophin complex is required for the function of myelin-forming Schwann cells. Similar to other tissues, the dystroglycan complex in Schwann cells localizes with different dystrophin family members in specific domains, thus promoting polarization. We show here that cleavage of dystroglycan by matrix metalloproteinases 2 and 9, an event that is considered pathological in most tissues, is finely and dynamically regulated in normal nerves and modulates dystroglycan complex composition and the size of Schwann cell compartments. In contrast, in nerves of Dy2j/2j mice, a model of laminin 211 deficiency, metalloproteinases 2 and 9 are increased, causing excessive dystroglycan cleavage and abnormal compartments. Pharmacological inhibition of cleavage rescues the cytoplasmic defects of Dy2j/2j Schwann cells. Thus, regulated cleavage may be a general mechanism to regulate protein complex composition in physiological conditions, whereas unregulated processing is pathogenic and a target for treatment in disease.

Original languageEnglish
Pages (from-to)12208-12217
Number of pages10
JournalJournal of Neuroscience
Volume31
Issue number34
DOIs
Publication statusPublished - Aug 24 2011

ASJC Scopus subject areas

  • Neuroscience(all)

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