Mn bioavailability by polarized Caco-2 cells

Comparison between Mn gluconate and Mn oxyprolinate

Chiara Foglieni, Mariangela Cavarelli, Mariarosaria Piscopiello, Alessandro Fulgenzi, Maria Ferrero

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Micronutrient inadequate intake is responsible of pathological deficiencies and there is a need of assessing the effectiveness of metal supplementation, frequently proposed to rebalance poor diets. Manganese (Mn) is present in many enzymatic intracellular systems crucial for the regulation of cell metabolism, and is contained in commercially available metal supplements. Methods. We compared the effects of two different commercial Mn forms, gluconate (MnGluc) and oxyprolinate (MnOxP). For this purpose we used the polarized Caco-2 cells cultured on transwell filters, an established in vitro model of intestinal epithelium. Since micronutrient deficiency may accelerate mitochondrial efficiency, the mitochondrial response of these cells, in the presence of MnGluc and MnOxP, by microscopy methods and by ATP luminescence assay was used. Results: In the presence of both MnOxP and MnGluc a sustained mitochondrial activity was shown by mitoTraker labeling (indicative of mitochondrial respiration), but ATP intracellular content remained comparable to untreated cells only in the presence of MnOxP. In addition MnOxP transiently up-regulated the antioxidant enzyme Mn superoxide dismutase more efficiently than MnGluc. Both metal treatments preserved NADH and NADPH diaphorase oxidative activity, avoided mitochondrial dysfunction, as assessed by the absence of a sustained phosphoERK activation, and were able to maintain cell viability. Conclusions: Collectively, our data indicate that MnOxP and MnGluc, and primarily the former, produce a moderate and safe modification of Caco-2 cell metabolism, by activating positive enzymatic mechanisms, thus could contribute to long-term maintenance of cell homeostasis.

Original languageEnglish
Article number77
JournalNutrition Journal
Volume10
Issue number1
DOIs
Publication statusPublished - 2011

Fingerprint

Caco-2 Cells
Manganese
Biological Availability
Micronutrients
Metals
Adenosine Triphosphate
Dihydrolipoamide Dehydrogenase
NADPH Dehydrogenase
Intestinal Mucosa
Luminescence
Superoxide Dismutase
Microscopy
Cell Survival
Respiration
Homeostasis
Antioxidants
gluconic acid
Diet
Enzymes

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Mn bioavailability by polarized Caco-2 cells : Comparison between Mn gluconate and Mn oxyprolinate. / Foglieni, Chiara; Cavarelli, Mariangela; Piscopiello, Mariarosaria; Fulgenzi, Alessandro; Ferrero, Maria.

In: Nutrition Journal, Vol. 10, No. 1, 77, 2011.

Research output: Contribution to journalArticle

Foglieni, Chiara ; Cavarelli, Mariangela ; Piscopiello, Mariarosaria ; Fulgenzi, Alessandro ; Ferrero, Maria. / Mn bioavailability by polarized Caco-2 cells : Comparison between Mn gluconate and Mn oxyprolinate. In: Nutrition Journal. 2011 ; Vol. 10, No. 1.
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