Mn(II) binding to human serum albumin: A 1H-NMR relaxometric study

Gabriella Fanali, Yu Cao, Paolo Ascenzi, Mauro Fasano

Research output: Contribution to journalArticle

Abstract

Human serum albumin (HSA) displays several metal binding sites, participating to essential and toxic metal ions disposal and transport. The major Zn(II) binding site, called Site A, is located at the I/II domain interface, with residues His67, Asn99, His247, and Asp249 contributing with five donor atoms to the metal ion coordination. Additionally, one water molecule takes part of the octahedral coordination geometry. The occurrence of the metal-coordinated water molecule allows the investigation of the metal complex geometry by water 1H-NMR relaxation, provided that the diamagnetic Zn(II) is replaced by the paramagnetic Mn(II). Here, the 1H-NMR relaxometric study of Mn(II) binding to HSA is reported. Mn(II) binding to HSA is modulated by Zn(II), pH, and myristate through competitive inhibition and allosteric mechanisms. The body of results indicates that the primary binding site of Zn(II) corresponds to the secondary binding site of Mn(II), i.e. the multimetal binding site A. Excess Zn(II) completely displaces Mn(II) from its primary site suggesting that the primary Mn(II) site corresponds to the secondary Zn(II) site. This uncharacterized site is functionally-linked to FA1; moreover, metal ion binding is modulated by myristate and pH. Noteworthy, water 1H-NMR relaxometry allowed a detailed analysis of thermodynamic properties of HSA-metal ion complexes.

Original languageEnglish
Pages (from-to)198-203
Number of pages6
JournalJournal of Inorganic Biochemistry
Volume117
DOIs
Publication statusPublished - Dec 2012

Fingerprint

Serum Albumin
Metals
Binding Sites
Nuclear magnetic resonance
Metal ions
Water
Coordination Complexes
Myristic Acid
Ions
Molecules
Geometry
Poisons
Ion Transport
Thermodynamics
Thermodynamic properties
Proton Magnetic Resonance Spectroscopy
Atoms

Keywords

  • H-NMR relaxometry
  • Human serum albumin
  • Ligand binding thermodynamics
  • Manganese
  • Metal binding
  • Zinc

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

Mn(II) binding to human serum albumin : A 1H-NMR relaxometric study. / Fanali, Gabriella; Cao, Yu; Ascenzi, Paolo; Fasano, Mauro.

In: Journal of Inorganic Biochemistry, Vol. 117, 12.2012, p. 198-203.

Research output: Contribution to journalArticle

Fanali, Gabriella ; Cao, Yu ; Ascenzi, Paolo ; Fasano, Mauro. / Mn(II) binding to human serum albumin : A 1H-NMR relaxometric study. In: Journal of Inorganic Biochemistry. 2012 ; Vol. 117. pp. 198-203.
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