Mobilization of capillary endothelium in vitro induced by effectors of angiogenesis in vivo

Giulio Alessandri, Katari Raju, Pietro M. Gullino

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

An assay to measure endothelial cell mobilization on a gelatin substratum has been developed. Utilization of the gelatin-agarose and Boyden chamber assays established that: (a) fragments or extracts of corneas treated with several effectors of angiogenesis in vivo acquired the capacity to mobilize the capillary endothelium in vitro; (b) this mobilization was selective for the capillary endothelium; endothelium from aorta and fibroblasts from human skin or rabbit cornea were unresponsive; and (c) among the effectors of angiogenesis utilized alone; i.e., without the intermediary action of the cornea, none were able to mobilize the capillary endothelium in vitro, except for the heparin-copper complex. The data are interpreted to indicate that new formation of capillaries in vivo is the end result of a cascade of events of which heparin and copper are important components.

Original languageEnglish
Pages (from-to)1790-1797
Number of pages8
JournalCancer Research
Volume43
Issue number4
Publication statusPublished - Apr 1 1983

Fingerprint

Vascular Endothelium
Cornea
Gelatin
Heparin
Copper
Sepharose
Endothelium
Aorta
Endothelial Cells
Fibroblasts
Rabbits
Skin
In Vitro Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Alessandri, G., Raju, K., & Gullino, P. M. (1983). Mobilization of capillary endothelium in vitro induced by effectors of angiogenesis in vivo. Cancer Research, 43(4), 1790-1797.

Mobilization of capillary endothelium in vitro induced by effectors of angiogenesis in vivo. / Alessandri, Giulio; Raju, Katari; Gullino, Pietro M.

In: Cancer Research, Vol. 43, No. 4, 01.04.1983, p. 1790-1797.

Research output: Contribution to journalArticle

Alessandri, G, Raju, K & Gullino, PM 1983, 'Mobilization of capillary endothelium in vitro induced by effectors of angiogenesis in vivo', Cancer Research, vol. 43, no. 4, pp. 1790-1797.
Alessandri, Giulio ; Raju, Katari ; Gullino, Pietro M. / Mobilization of capillary endothelium in vitro induced by effectors of angiogenesis in vivo. In: Cancer Research. 1983 ; Vol. 43, No. 4. pp. 1790-1797.
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