Modeling of DNA fragmentation induced in human fibroblasts by 56Fe ions

D. Alloni, F. Ballarini, M. Belli, A. Campa, G. Esposito, W. Friedland, M. Liotta, A. Ottolenghi, H. G. Paretzke

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In this work we have compared the pattern of DNA fragmentation (in the size range 1-5700 kbp) induced in human fibroblasts by γ-rays with that induced by Fe ions with an energy of 115 MeV/u; Fe ions are considered of biological significance for radiation protection issues during long term astronauts' travels. The study has taken into account the comparison of the experimental fragmentation spectra, their analysis performed through the implementation of a phenomenological model, and Monte Carlo simulations performed with the PARTRAC code. The phenomenological method characterizes in an approximate but simple way the nonrandom nature of the experimental fragment distribution caused by high LET radiation; it has the advantage to take into account in a detailed way the background fragmentation. The PARTRAC simulations, on the other hand, thanks to the accurate representation of the chromatin geometry and of the physical and physico-chemical processes associated with the energy deposition by radiation, offer the possibility to compute the spectra and to compare them with the experimental data. We found a satisfactory agreement between the experimental data and the results obtained with PARTRAC, recently upgraded with the implementation of ions heavier than alpha particles; this agreement represents a first validation of the code. A relevant result is represented by the very high number of fragments that, according to the Monte Carlo simulations, are produced by iron ions in the size range

Original languageEnglish
Pages (from-to)1401-1407
Number of pages7
JournalAdvances in Space Research
Volume40
Issue number9
DOIs
Publication statusPublished - 2007

Fingerprint

fibroblasts
Fibroblasts
fragmentation
DNA
deoxyribonucleic acid
ion
Ions
fragments
range size
modeling
Radiation
Radiation protection
chromatin
ions
astronauts
Alpha particles
simulation
radiation protection
radiation
Heavy ions

Keywords

  • DNA fragmentation
  • Iron ions
  • Monte Carlo simulations
  • Nonrandom DSB distributions
  • RBE for DSB yield

ASJC Scopus subject areas

  • Space and Planetary Science
  • Astronomy and Astrophysics

Cite this

Alloni, D., Ballarini, F., Belli, M., Campa, A., Esposito, G., Friedland, W., ... Paretzke, H. G. (2007). Modeling of DNA fragmentation induced in human fibroblasts by 56Fe ions. Advances in Space Research, 40(9), 1401-1407. https://doi.org/10.1016/j.asr.2007.05.078

Modeling of DNA fragmentation induced in human fibroblasts by 56Fe ions. / Alloni, D.; Ballarini, F.; Belli, M.; Campa, A.; Esposito, G.; Friedland, W.; Liotta, M.; Ottolenghi, A.; Paretzke, H. G.

In: Advances in Space Research, Vol. 40, No. 9, 2007, p. 1401-1407.

Research output: Contribution to journalArticle

Alloni, D, Ballarini, F, Belli, M, Campa, A, Esposito, G, Friedland, W, Liotta, M, Ottolenghi, A & Paretzke, HG 2007, 'Modeling of DNA fragmentation induced in human fibroblasts by 56Fe ions', Advances in Space Research, vol. 40, no. 9, pp. 1401-1407. https://doi.org/10.1016/j.asr.2007.05.078
Alloni D, Ballarini F, Belli M, Campa A, Esposito G, Friedland W et al. Modeling of DNA fragmentation induced in human fibroblasts by 56Fe ions. Advances in Space Research. 2007;40(9):1401-1407. https://doi.org/10.1016/j.asr.2007.05.078
Alloni, D. ; Ballarini, F. ; Belli, M. ; Campa, A. ; Esposito, G. ; Friedland, W. ; Liotta, M. ; Ottolenghi, A. ; Paretzke, H. G. / Modeling of DNA fragmentation induced in human fibroblasts by 56Fe ions. In: Advances in Space Research. 2007 ; Vol. 40, No. 9. pp. 1401-1407.
@article{8c80c5d0df134f93831c5d58fa7b3ea8,
title = "Modeling of DNA fragmentation induced in human fibroblasts by 56Fe ions",
abstract = "In this work we have compared the pattern of DNA fragmentation (in the size range 1-5700 kbp) induced in human fibroblasts by γ-rays with that induced by Fe ions with an energy of 115 MeV/u; Fe ions are considered of biological significance for radiation protection issues during long term astronauts' travels. The study has taken into account the comparison of the experimental fragmentation spectra, their analysis performed through the implementation of a phenomenological model, and Monte Carlo simulations performed with the PARTRAC code. The phenomenological method characterizes in an approximate but simple way the nonrandom nature of the experimental fragment distribution caused by high LET radiation; it has the advantage to take into account in a detailed way the background fragmentation. The PARTRAC simulations, on the other hand, thanks to the accurate representation of the chromatin geometry and of the physical and physico-chemical processes associated with the energy deposition by radiation, offer the possibility to compute the spectra and to compare them with the experimental data. We found a satisfactory agreement between the experimental data and the results obtained with PARTRAC, recently upgraded with the implementation of ions heavier than alpha particles; this agreement represents a first validation of the code. A relevant result is represented by the very high number of fragments that, according to the Monte Carlo simulations, are produced by iron ions in the size range",
keywords = "DNA fragmentation, Iron ions, Monte Carlo simulations, Nonrandom DSB distributions, RBE for DSB yield",
author = "D. Alloni and F. Ballarini and M. Belli and A. Campa and G. Esposito and W. Friedland and M. Liotta and A. Ottolenghi and Paretzke, {H. G.}",
year = "2007",
doi = "10.1016/j.asr.2007.05.078",
language = "English",
volume = "40",
pages = "1401--1407",
journal = "Advances in Space Research",
issn = "0273-1177",
publisher = "Elsevier Limited",
number = "9",

}

TY - JOUR

T1 - Modeling of DNA fragmentation induced in human fibroblasts by 56Fe ions

AU - Alloni, D.

AU - Ballarini, F.

AU - Belli, M.

AU - Campa, A.

AU - Esposito, G.

AU - Friedland, W.

AU - Liotta, M.

AU - Ottolenghi, A.

AU - Paretzke, H. G.

PY - 2007

Y1 - 2007

N2 - In this work we have compared the pattern of DNA fragmentation (in the size range 1-5700 kbp) induced in human fibroblasts by γ-rays with that induced by Fe ions with an energy of 115 MeV/u; Fe ions are considered of biological significance for radiation protection issues during long term astronauts' travels. The study has taken into account the comparison of the experimental fragmentation spectra, their analysis performed through the implementation of a phenomenological model, and Monte Carlo simulations performed with the PARTRAC code. The phenomenological method characterizes in an approximate but simple way the nonrandom nature of the experimental fragment distribution caused by high LET radiation; it has the advantage to take into account in a detailed way the background fragmentation. The PARTRAC simulations, on the other hand, thanks to the accurate representation of the chromatin geometry and of the physical and physico-chemical processes associated with the energy deposition by radiation, offer the possibility to compute the spectra and to compare them with the experimental data. We found a satisfactory agreement between the experimental data and the results obtained with PARTRAC, recently upgraded with the implementation of ions heavier than alpha particles; this agreement represents a first validation of the code. A relevant result is represented by the very high number of fragments that, according to the Monte Carlo simulations, are produced by iron ions in the size range

AB - In this work we have compared the pattern of DNA fragmentation (in the size range 1-5700 kbp) induced in human fibroblasts by γ-rays with that induced by Fe ions with an energy of 115 MeV/u; Fe ions are considered of biological significance for radiation protection issues during long term astronauts' travels. The study has taken into account the comparison of the experimental fragmentation spectra, their analysis performed through the implementation of a phenomenological model, and Monte Carlo simulations performed with the PARTRAC code. The phenomenological method characterizes in an approximate but simple way the nonrandom nature of the experimental fragment distribution caused by high LET radiation; it has the advantage to take into account in a detailed way the background fragmentation. The PARTRAC simulations, on the other hand, thanks to the accurate representation of the chromatin geometry and of the physical and physico-chemical processes associated with the energy deposition by radiation, offer the possibility to compute the spectra and to compare them with the experimental data. We found a satisfactory agreement between the experimental data and the results obtained with PARTRAC, recently upgraded with the implementation of ions heavier than alpha particles; this agreement represents a first validation of the code. A relevant result is represented by the very high number of fragments that, according to the Monte Carlo simulations, are produced by iron ions in the size range

KW - DNA fragmentation

KW - Iron ions

KW - Monte Carlo simulations

KW - Nonrandom DSB distributions

KW - RBE for DSB yield

UR - http://www.scopus.com/inward/record.url?scp=34948818285&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34948818285&partnerID=8YFLogxK

U2 - 10.1016/j.asr.2007.05.078

DO - 10.1016/j.asr.2007.05.078

M3 - Article

AN - SCOPUS:34948818285

VL - 40

SP - 1401

EP - 1407

JO - Advances in Space Research

JF - Advances in Space Research

SN - 0273-1177

IS - 9

ER -