Modeling of fibrin gels based on confocal microscopy and light-scattering data

Davide Magatti, Matteo Molteni, Barbara Cardinali, Mattia Rocco, Fabio Ferri

Research output: Contribution to journalArticlepeer-review

Abstract

Fibrin gels are biological networks that play a fundamental role in blood coagulation and other patho/physiological processes, such as thrombosis and cancer. Electron and confocal microscopies show a collection of fibers that are relatively monodisperse in diameter, not uniformly distributed, and connected at nodal points with a branching order of ∼3-4. Although in the confocal images the hydrated fibers appear to be quite straight (mass fractal dimension Dm = 1), for the overall system 1m0 between their centers of mass so that they are overlapped by a factor η = ξ/ξ0 and have Dm ∼1.2-1.6. The in silico gels' structure is quantitatively analyzed by its 3D spatial correlation function g3D(r) and corresponding power spectrum I(q) = FFT3D[g3D(r)], from which ρ, d, Dm, η, and ξ0 can be extracted. In particular, ξ0 provides an excellent estimate of the gel mesh size. The in silico gels' I(q) compares quite well with real gels' elastic light-scattering measurements. We then derived an analytical form factor for accurately fitting the scattering data, which allowed us to directly recover the gels' structural parameters.

Original languageEnglish
Pages (from-to)1151-1159
Number of pages9
JournalBiophysical Journal
Volume104
Issue number5
DOIs
Publication statusPublished - Mar 5 2013

ASJC Scopus subject areas

  • Biophysics

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