Models and methods for conditioning the ischemic brain

Antonio Vinciguerra, Ornella Cuomo, Pasquale Cepparulo, Serenella Anzilotti, Paola Brancaccio, Rossana Sirabella, Natascia Guida, Lucio Annunziato, Giuseppe Pignataro

Research output: Contribution to journalArticle

Abstract

Background: In the last decades the need to find new neuroprotective targets has addressed the researchers to investigate the endogenous molecular mechanisms that brain activates when exposed to a conditioning stimulus. Indeed, conditioning is an adaptive biological process activated by those interventions able to confer resistance to a deleterious brain event through the exposure to a sub-threshold insult. Specifically, preconditioning and postconditioning are realized when the conditioning stimulus is applied before or after, respectively, the harmul ischemia. Aims and Results: The present review will describe the most common methods to induce brain conditioning, with particular regards to surgical, physical exercise, temperature-induced and pharmacological approaches. It has been well recognized that when the subliminal stimulus is delivered after the ischemic insult, the achieved neuroprotection is comparable to that observed in models of ischemic preconditioning. In addition, subjecting the brain to both preconditioning as well as postconditioning did not cause greater protection than each treatment alone. Conclusions: The last decades have provided fascinating insights into the mechanisms and potential application of strategies to induce brain conditioning. Since the identification of intrinsic cell‐survival pathways should provide more direct opportunities for translational neuroprotection trials, an accurate examination of the different models of preconditioning and postconditioning is mandatory before starting any new project.

Original languageEnglish
Pages (from-to)63-74
Number of pages12
JournalJournal of Neuroscience Methods
Volume310
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Brain
Biological Phenomena
Ischemic Preconditioning
Ischemia
Research Personnel
Pharmacology
Exercise
Temperature
Neuroprotection
Therapeutics

Keywords

  • Brain ischemia
  • Neuroprotection
  • Postconditioning
  • Preconditioning
  • Stroke

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Vinciguerra, A., Cuomo, O., Cepparulo, P., Anzilotti, S., Brancaccio, P., Sirabella, R., ... Pignataro, G. (2018). Models and methods for conditioning the ischemic brain. Journal of Neuroscience Methods, 310, 63-74. https://doi.org/10.1016/j.jneumeth.2018.09.029

Models and methods for conditioning the ischemic brain. / Vinciguerra, Antonio; Cuomo, Ornella; Cepparulo, Pasquale; Anzilotti, Serenella; Brancaccio, Paola; Sirabella, Rossana; Guida, Natascia; Annunziato, Lucio; Pignataro, Giuseppe.

In: Journal of Neuroscience Methods, Vol. 310, 01.12.2018, p. 63-74.

Research output: Contribution to journalArticle

Vinciguerra, A, Cuomo, O, Cepparulo, P, Anzilotti, S, Brancaccio, P, Sirabella, R, Guida, N, Annunziato, L & Pignataro, G 2018, 'Models and methods for conditioning the ischemic brain', Journal of Neuroscience Methods, vol. 310, pp. 63-74. https://doi.org/10.1016/j.jneumeth.2018.09.029
Vinciguerra A, Cuomo O, Cepparulo P, Anzilotti S, Brancaccio P, Sirabella R et al. Models and methods for conditioning the ischemic brain. Journal of Neuroscience Methods. 2018 Dec 1;310:63-74. https://doi.org/10.1016/j.jneumeth.2018.09.029
Vinciguerra, Antonio ; Cuomo, Ornella ; Cepparulo, Pasquale ; Anzilotti, Serenella ; Brancaccio, Paola ; Sirabella, Rossana ; Guida, Natascia ; Annunziato, Lucio ; Pignataro, Giuseppe. / Models and methods for conditioning the ischemic brain. In: Journal of Neuroscience Methods. 2018 ; Vol. 310. pp. 63-74.
@article{d3f22d9ddfc14f0faaf8a9d62e063962,
title = "Models and methods for conditioning the ischemic brain",
abstract = "Background: In the last decades the need to find new neuroprotective targets has addressed the researchers to investigate the endogenous molecular mechanisms that brain activates when exposed to a conditioning stimulus. Indeed, conditioning is an adaptive biological process activated by those interventions able to confer resistance to a deleterious brain event through the exposure to a sub-threshold insult. Specifically, preconditioning and postconditioning are realized when the conditioning stimulus is applied before or after, respectively, the harmul ischemia. Aims and Results: The present review will describe the most common methods to induce brain conditioning, with particular regards to surgical, physical exercise, temperature-induced and pharmacological approaches. It has been well recognized that when the subliminal stimulus is delivered after the ischemic insult, the achieved neuroprotection is comparable to that observed in models of ischemic preconditioning. In addition, subjecting the brain to both preconditioning as well as postconditioning did not cause greater protection than each treatment alone. Conclusions: The last decades have provided fascinating insights into the mechanisms and potential application of strategies to induce brain conditioning. Since the identification of intrinsic cell‐survival pathways should provide more direct opportunities for translational neuroprotection trials, an accurate examination of the different models of preconditioning and postconditioning is mandatory before starting any new project.",
keywords = "Brain ischemia, Neuroprotection, Postconditioning, Preconditioning, Stroke",
author = "Antonio Vinciguerra and Ornella Cuomo and Pasquale Cepparulo and Serenella Anzilotti and Paola Brancaccio and Rossana Sirabella and Natascia Guida and Lucio Annunziato and Giuseppe Pignataro",
year = "2018",
month = "12",
day = "1",
doi = "10.1016/j.jneumeth.2018.09.029",
language = "English",
volume = "310",
pages = "63--74",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",

}

TY - JOUR

T1 - Models and methods for conditioning the ischemic brain

AU - Vinciguerra, Antonio

AU - Cuomo, Ornella

AU - Cepparulo, Pasquale

AU - Anzilotti, Serenella

AU - Brancaccio, Paola

AU - Sirabella, Rossana

AU - Guida, Natascia

AU - Annunziato, Lucio

AU - Pignataro, Giuseppe

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Background: In the last decades the need to find new neuroprotective targets has addressed the researchers to investigate the endogenous molecular mechanisms that brain activates when exposed to a conditioning stimulus. Indeed, conditioning is an adaptive biological process activated by those interventions able to confer resistance to a deleterious brain event through the exposure to a sub-threshold insult. Specifically, preconditioning and postconditioning are realized when the conditioning stimulus is applied before or after, respectively, the harmul ischemia. Aims and Results: The present review will describe the most common methods to induce brain conditioning, with particular regards to surgical, physical exercise, temperature-induced and pharmacological approaches. It has been well recognized that when the subliminal stimulus is delivered after the ischemic insult, the achieved neuroprotection is comparable to that observed in models of ischemic preconditioning. In addition, subjecting the brain to both preconditioning as well as postconditioning did not cause greater protection than each treatment alone. Conclusions: The last decades have provided fascinating insights into the mechanisms and potential application of strategies to induce brain conditioning. Since the identification of intrinsic cell‐survival pathways should provide more direct opportunities for translational neuroprotection trials, an accurate examination of the different models of preconditioning and postconditioning is mandatory before starting any new project.

AB - Background: In the last decades the need to find new neuroprotective targets has addressed the researchers to investigate the endogenous molecular mechanisms that brain activates when exposed to a conditioning stimulus. Indeed, conditioning is an adaptive biological process activated by those interventions able to confer resistance to a deleterious brain event through the exposure to a sub-threshold insult. Specifically, preconditioning and postconditioning are realized when the conditioning stimulus is applied before or after, respectively, the harmul ischemia. Aims and Results: The present review will describe the most common methods to induce brain conditioning, with particular regards to surgical, physical exercise, temperature-induced and pharmacological approaches. It has been well recognized that when the subliminal stimulus is delivered after the ischemic insult, the achieved neuroprotection is comparable to that observed in models of ischemic preconditioning. In addition, subjecting the brain to both preconditioning as well as postconditioning did not cause greater protection than each treatment alone. Conclusions: The last decades have provided fascinating insights into the mechanisms and potential application of strategies to induce brain conditioning. Since the identification of intrinsic cell‐survival pathways should provide more direct opportunities for translational neuroprotection trials, an accurate examination of the different models of preconditioning and postconditioning is mandatory before starting any new project.

KW - Brain ischemia

KW - Neuroprotection

KW - Postconditioning

KW - Preconditioning

KW - Stroke

UR - http://www.scopus.com/inward/record.url?scp=85054662172&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85054662172&partnerID=8YFLogxK

U2 - 10.1016/j.jneumeth.2018.09.029

DO - 10.1016/j.jneumeth.2018.09.029

M3 - Article

C2 - 30287283

AN - SCOPUS:85054662172

VL - 310

SP - 63

EP - 74

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

ER -