Modern methods to investigate the oligomerization of glycoprotein hormone receptors (TSHR, LHR, FSHR)

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

As for other GPCRs, the oligomerization of glycoprotein hormone receptors (GPHRs) appears as critical event for receptor function. By means of modern techniques based on the BRET or FRET principle, GPHR oligomerization has been reported to explain several physiological and pathological conditions. In particular, the presence of oligomers was demonstrated not only in in vitro heterologous systems but also in in vivo tissues, and GPHR homodimerization appears associated with strong negative cooperativity, thus suggesting that one hormone molecule may be sufficient for receptor dimer stimulation. In addition, oligomerization has been reported to occur early during the posttranslational maturation process and to be involved in the dominant negative effect exerted by loss-of-function TSH receptor (TSHR) mutants, that are prevalently retained inside the cell, on the surface expression of wild-type receptors. This molecular mechanism thus explains the dominant inheritance of certain forms of TSH resistance. Here, we provide the description of the methods used in the original BRET, FRET, and HTRF-RET experiments.

Original languageEnglish
Title of host publicationMethods in Enzymology
Pages367-383
Number of pages17
Volume521
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Enzymology
Volume521
ISSN (Print)00766879
ISSN (Electronic)15577988

Keywords

  • BRET
  • Dominant negative effect
  • FRET
  • FSHR
  • Glycoprotein hormone receptors
  • HTRF-RET
  • LHR
  • Negative cooperativity
  • TSHR

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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