Abstract
Human bronchial epithelial cells were treated in vitro with interferon- γ or tumor necrosis factor-α to assess their effect on transepithelial ion transport. Short-circuit current measurements revealed that Na+ absorption was markedly inhibited by interferon-γ (10-1,000 U/ml). The cystic fibrosis transmembrane conductance regulator was also downregulated by interferon-γ as evident at the protein level and by the decrease in the cAMP-dependent current. On the other hand, interferon-γ caused an increase of the current elicited by apical UTP application, which is due to the activity of Ca2+- dependent Cl- channels. Tumor necrosis factor-α caused few changes in ion transport. Transepithelial fluid transport was measured in normal and cystic fibrosis cells. At rest, both types of cells showed an amiloride-sensitive fluid absorption that was inhibited by interferon-γ but not by tumor necrosis factor-α. Our results show that interferon-γ alters the transepithelial ion transport of cultured bronchial cells. This effect may change the ion composition and/or volume of periciliary fluid.
| Original language | English |
|---|---|
| Journal | American Journal of Physiology - Lung Cellular and Molecular Physiology |
| Volume | 278 |
| Issue number | 6 22-6 |
| Publication status | Published - Jun 2000 |
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Keywords
- Airway epithelium
- Airway surface fluid
- Chloride transport
- Cystic fibrosis
- Sodium absorption
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
- Cell Biology
- Physiology
- Physiology (medical)
Cite this
Modification of transepithelial ion transport in human cultured bronchial epithelial cells by interferon-γ. / Galietta, Luis J V; Folli, Chiara; Marchetti, Carla; Romano, Luca; Carpani, Daniela; Conese, Massimo; Zegarra-Moran, Olga.
In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 278, No. 6 22-6, 06.2000.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Modification of transepithelial ion transport in human cultured bronchial epithelial cells by interferon-γ
AU - Galietta, Luis J V
AU - Folli, Chiara
AU - Marchetti, Carla
AU - Romano, Luca
AU - Carpani, Daniela
AU - Conese, Massimo
AU - Zegarra-Moran, Olga
PY - 2000/6
Y1 - 2000/6
N2 - Human bronchial epithelial cells were treated in vitro with interferon- γ or tumor necrosis factor-α to assess their effect on transepithelial ion transport. Short-circuit current measurements revealed that Na+ absorption was markedly inhibited by interferon-γ (10-1,000 U/ml). The cystic fibrosis transmembrane conductance regulator was also downregulated by interferon-γ as evident at the protein level and by the decrease in the cAMP-dependent current. On the other hand, interferon-γ caused an increase of the current elicited by apical UTP application, which is due to the activity of Ca2+- dependent Cl- channels. Tumor necrosis factor-α caused few changes in ion transport. Transepithelial fluid transport was measured in normal and cystic fibrosis cells. At rest, both types of cells showed an amiloride-sensitive fluid absorption that was inhibited by interferon-γ but not by tumor necrosis factor-α. Our results show that interferon-γ alters the transepithelial ion transport of cultured bronchial cells. This effect may change the ion composition and/or volume of periciliary fluid.
AB - Human bronchial epithelial cells were treated in vitro with interferon- γ or tumor necrosis factor-α to assess their effect on transepithelial ion transport. Short-circuit current measurements revealed that Na+ absorption was markedly inhibited by interferon-γ (10-1,000 U/ml). The cystic fibrosis transmembrane conductance regulator was also downregulated by interferon-γ as evident at the protein level and by the decrease in the cAMP-dependent current. On the other hand, interferon-γ caused an increase of the current elicited by apical UTP application, which is due to the activity of Ca2+- dependent Cl- channels. Tumor necrosis factor-α caused few changes in ion transport. Transepithelial fluid transport was measured in normal and cystic fibrosis cells. At rest, both types of cells showed an amiloride-sensitive fluid absorption that was inhibited by interferon-γ but not by tumor necrosis factor-α. Our results show that interferon-γ alters the transepithelial ion transport of cultured bronchial cells. This effect may change the ion composition and/or volume of periciliary fluid.
KW - Airway epithelium
KW - Airway surface fluid
KW - Chloride transport
KW - Cystic fibrosis
KW - Sodium absorption
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UR - http://www.scopus.com/inward/citedby.url?scp=0033946122&partnerID=8YFLogxK
M3 - Article
C2 - 10835324
AN - SCOPUS:0033946122
VL - 278
JO - American Journal of Physiology
JF - American Journal of Physiology
SN - 0363-6119
IS - 6 22-6
ER -