Modification of transepithelial ion transport in human cultured bronchial epithelial cells by interferon-γ

Luis J V Galietta, Chiara Folli, Carla Marchetti, Luca Romano, Daniela Carpani, Massimo Conese, Olga Zegarra-Moran

Research output: Contribution to journalArticle

Abstract

Human bronchial epithelial cells were treated in vitro with interferon- γ or tumor necrosis factor-α to assess their effect on transepithelial ion transport. Short-circuit current measurements revealed that Na+ absorption was markedly inhibited by interferon-γ (10-1,000 U/ml). The cystic fibrosis transmembrane conductance regulator was also downregulated by interferon-γ as evident at the protein level and by the decrease in the cAMP-dependent current. On the other hand, interferon-γ caused an increase of the current elicited by apical UTP application, which is due to the activity of Ca2+- dependent Cl- channels. Tumor necrosis factor-α caused few changes in ion transport. Transepithelial fluid transport was measured in normal and cystic fibrosis cells. At rest, both types of cells showed an amiloride-sensitive fluid absorption that was inhibited by interferon-γ but not by tumor necrosis factor-α. Our results show that interferon-γ alters the transepithelial ion transport of cultured bronchial cells. This effect may change the ion composition and/or volume of periciliary fluid.

Original languageEnglish
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume278
Issue number6 22-6
Publication statusPublished - Jun 2000

Fingerprint

Ion Transport
Interferons
Epithelial Cells
Tumor Necrosis Factor-alpha
Cystic Fibrosis Transmembrane Conductance Regulator
Uridine Triphosphate
Amiloride
Cystic Fibrosis
Cultured Cells
Down-Regulation
Ions
Proteins

Keywords

  • Airway epithelium
  • Airway surface fluid
  • Chloride transport
  • Cystic fibrosis
  • Sodium absorption

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Galietta, L. J. V., Folli, C., Marchetti, C., Romano, L., Carpani, D., Conese, M., & Zegarra-Moran, O. (2000). Modification of transepithelial ion transport in human cultured bronchial epithelial cells by interferon-γ. American Journal of Physiology - Lung Cellular and Molecular Physiology, 278(6 22-6).

Modification of transepithelial ion transport in human cultured bronchial epithelial cells by interferon-γ. / Galietta, Luis J V; Folli, Chiara; Marchetti, Carla; Romano, Luca; Carpani, Daniela; Conese, Massimo; Zegarra-Moran, Olga.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 278, No. 6 22-6, 06.2000.

Research output: Contribution to journalArticle

Galietta, LJV, Folli, C, Marchetti, C, Romano, L, Carpani, D, Conese, M & Zegarra-Moran, O 2000, 'Modification of transepithelial ion transport in human cultured bronchial epithelial cells by interferon-γ', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 278, no. 6 22-6.
Galietta, Luis J V ; Folli, Chiara ; Marchetti, Carla ; Romano, Luca ; Carpani, Daniela ; Conese, Massimo ; Zegarra-Moran, Olga. / Modification of transepithelial ion transport in human cultured bronchial epithelial cells by interferon-γ. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2000 ; Vol. 278, No. 6 22-6.
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