Modifications at C(5) of 2-(2-Pyrrolidinyl)-Substituted 1,4-Benzodioxane Elicit Potent α4β2 Nicotinic Acetylcholine Receptor Partial Agonism with High Selectivity over the α3β4 Subtype

Francesco Bavo, Marco Pallavicini, Cecilia Gotti, Rebecca Appiani, Milena Moretti, Sara Francesca Colombo, Susanna Pucci, Paola Viani, Roberta Budriesi, Massimiliano Renzi, Sergio Fucile, Cristiano Bolchi

Research output: Contribution to journalArticlepeer-review

Abstract

A series of diastereomeric 2-(2-pyrrolidinyl)-1,4-benzodioxanes bearing a small, hydrogen-bonding substituent at the 7-, 6-, or 5-position of benzodioxane have been studied for α4β2 and α3β4 nicotinic acetylcholine receptor affinity and activity. Analogous to C(5)H replacement with N and to a much greater extent than decoration at C(7), substitution at benzodioxane C(5) confers very high α4β2/α3β4 selectivity to the α4β2 partial agonism. Docking into the two receptor structures recently determined by cryo-electron microscopy and site-directed mutagenesis at the minus β2 side converge in indicating that the limited accommodation capacity of the β2 pocket, compared to that of the β4 pocket, makes substitution at C(5) rather than at more projecting C(7) position determinant for this pursued subtype selectivity.

Original languageEnglish
Pages (from-to)15668-15692
Number of pages25
JournalJournal of Medicinal Chemistry
Volume63
Issue number24
DOIs
Publication statusPublished - Dec 24 2020

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