Modifications of functional and physico-chemical properties of rat ileal plasma membranes

G. Gaggiotti, M. Taus, L. Spazzafumo, M. Tesei, R. La Rocca, L. Mazzanti

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A membrane fraction enriched with plasma membranes was isolated from rat ileal brush-border cells before and after five-day starvation of the animals. Cholesterol/phospholipid ratio of the standard cell membranes decreased highly significantly (0.42 to 0.18), as did the microviscosity of the membranes determined by polarization of fluorescence (0.187 to 0.142). Concomitantly, the specific activity of Na,K-ATPase in the basolateral membranes significantly increased (59 to 83 μmol ATP hydrolyzed per mg protein per min).

Original languageEnglish
Pages (from-to)851-854
Number of pages4
JournalBiochemistry and Molecular Biology International
Volume35
Issue number4
Publication statusPublished - 1995

Fingerprint

Cell membranes
Chemical properties
Rats
Cell Membrane
Membranes
Fluorescence Polarization
Brushes
Microvilli
Starvation
Adenosine Triphosphatases
Phospholipids
Animals
Adenosine Triphosphate
Fluorescence
Cholesterol
Polarization
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Modifications of functional and physico-chemical properties of rat ileal plasma membranes. / Gaggiotti, G.; Taus, M.; Spazzafumo, L.; Tesei, M.; La Rocca, R.; Mazzanti, L.

In: Biochemistry and Molecular Biology International, Vol. 35, No. 4, 1995, p. 851-854.

Research output: Contribution to journalArticle

Gaggiotti, G. ; Taus, M. ; Spazzafumo, L. ; Tesei, M. ; La Rocca, R. ; Mazzanti, L. / Modifications of functional and physico-chemical properties of rat ileal plasma membranes. In: Biochemistry and Molecular Biology International. 1995 ; Vol. 35, No. 4. pp. 851-854.
@article{4471a6b313a041ea9e93590465dd7864,
title = "Modifications of functional and physico-chemical properties of rat ileal plasma membranes",
abstract = "A membrane fraction enriched with plasma membranes was isolated from rat ileal brush-border cells before and after five-day starvation of the animals. Cholesterol/phospholipid ratio of the standard cell membranes decreased highly significantly (0.42 to 0.18), as did the microviscosity of the membranes determined by polarization of fluorescence (0.187 to 0.142). Concomitantly, the specific activity of Na,K-ATPase in the basolateral membranes significantly increased (59 to 83 μmol ATP hydrolyzed per mg protein per min).",
author = "G. Gaggiotti and M. Taus and L. Spazzafumo and M. Tesei and {La Rocca}, R. and L. Mazzanti",
year = "1995",
language = "English",
volume = "35",
pages = "851--854",
journal = "Biochemistry and Molecular Biology International",
issn = "1039-9712",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Modifications of functional and physico-chemical properties of rat ileal plasma membranes

AU - Gaggiotti, G.

AU - Taus, M.

AU - Spazzafumo, L.

AU - Tesei, M.

AU - La Rocca, R.

AU - Mazzanti, L.

PY - 1995

Y1 - 1995

N2 - A membrane fraction enriched with plasma membranes was isolated from rat ileal brush-border cells before and after five-day starvation of the animals. Cholesterol/phospholipid ratio of the standard cell membranes decreased highly significantly (0.42 to 0.18), as did the microviscosity of the membranes determined by polarization of fluorescence (0.187 to 0.142). Concomitantly, the specific activity of Na,K-ATPase in the basolateral membranes significantly increased (59 to 83 μmol ATP hydrolyzed per mg protein per min).

AB - A membrane fraction enriched with plasma membranes was isolated from rat ileal brush-border cells before and after five-day starvation of the animals. Cholesterol/phospholipid ratio of the standard cell membranes decreased highly significantly (0.42 to 0.18), as did the microviscosity of the membranes determined by polarization of fluorescence (0.187 to 0.142). Concomitantly, the specific activity of Na,K-ATPase in the basolateral membranes significantly increased (59 to 83 μmol ATP hydrolyzed per mg protein per min).

UR - http://www.scopus.com/inward/record.url?scp=0029554763&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029554763&partnerID=8YFLogxK

M3 - Article

VL - 35

SP - 851

EP - 854

JO - Biochemistry and Molecular Biology International

JF - Biochemistry and Molecular Biology International

SN - 1039-9712

IS - 4

ER -