Modifications of HIV-1 DNA and provirus-infected cells during 24 months of intermittent highly active antiretroviral therapy

Lucia Palmisano, Marina Giuliano, Flavia Chiarotti, Marisa Zanchetta, Mauro Andreotti, Maria F. Pirillo, Elisabetta Riva, Guido Antonelli, Anita De Rossi, Stefano Vella

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Few data have been reported on the dynamics of HIV-1 DNA during intermittent highly active antiretroviral therapy (HAART). In this study, we measured cell-associated HIV-1 DNA and provirus-infected cells during the Istituto Superiore di Sanità-Pulsed Antiretroviral Therapy (ISS-PART) clinical trial. METHODS: HIV-1 DNA was measured by real-time polymerase chain reaction (PCR) in the peripheral blood mononuclear cells (PBMCs) of 37 subjects enrolled in the ISS-PART, a randomized clinical trial comparing 24 months of intermittent (arm B) versus continuous (arm A) HAART in chronic HIV infection. In 14 subjects, the number of provirus-infected cells was also measured at baseline and at month 24. RESULTS: At baseline, the number of HIV-1 DNA copies/10 PBMCs was similar in arm B (mean ± SD: 121 ± 172, median = 35) and arm A (mean ± SD: 107 ± 153, median = 10) (P = not significant [n.s.]). No significant variations occurred over time; at 24 months, the HIV-1 DNA level was 77 ± 28 (median = 30) copies/10 PBMCs in arm B and 166 ± 321 copies/10 PBMCs (median = 10) in arm A (P = n.s.). At baseline, the provirus-infected cell counts were 85 ± 98 (median = 50) cells/10 PBMCs in arm B and 92 ± 113 (median = 50) cells/10 PBMCs in arm A (P = n.s.), with no variations at 24 months. CONCLUSIONS: These findings suggest that the intermittent schedule of the ISS-PART has no major impact on viral reservoirs, at least in a midterm follow-up.

Original languageEnglish
Pages (from-to)68-71
Number of pages4
JournalJournal of Acquired Immune Deficiency Syndromes
Volume48
Issue number1
DOIs
Publication statusPublished - May 2008

Keywords

  • Highly active antiretroviral therapy
  • HIV-1 DNA
  • Treatment interruptions

ASJC Scopus subject areas

  • Virology
  • Immunology

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