Modifications of proteoglycans extracted from monolayer cultures of young and senescent human skin fibroblasts

Alberto Passi, Riccardo Albertini, Francesco Campagnari, Giancarlo De Luca

Research output: Contribution to journalArticle

Abstract

Proteoglycans (PGs) were extracted from culture monolayers of human skin fibroblasts (HFs) at early and late passages. Total PGs from senescent cells had markedly reduced abilities to bind type I collagen and hyaluronic acid, but retained normal binding properties with fibronectin and laminin. The constituent polysaccharidcs of PGs were comparatively characterised. PGs recovered from young and senescent HF cultures had equivalent total polyanionic charges and similar size distributions of the glycosaminoglycan chains. This applied to both types of polysaccharide chains found in PGs, namely the galactosaminoglycuronans (GalN-GAGs) and the glucosaminoglycuronans (GlcN-GAGs). However, senescent HFs produced a greater proportion of PGs containing GlcN-GAG chains and increased the sulphation of the remaining PG fraction with GnlN-GAG moieties, yielding a major gain of C6-sulphate groups in the galactosamine residues.

Original languageEnglish
Pages (from-to)175-178
Number of pages4
JournalFEBS Letters
Volume420
Issue number2-3
DOIs
Publication statusPublished - Dec 29 1997

Keywords

  • Culture monolayer
  • Fibroblast
  • Glycosaminoglycan
  • Proteoglycan
  • Replicative senescence

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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