Modifications of proteoglycans produced by human skin fibroblast cultures during replicative senescence

A. Passi, R. Albertini, A. Bardoni, S. Rindi, R. Salvini, G. Pallavicini, G. De Luca

Research output: Contribution to journalArticle


The properties of proteoglycans (PGs) produced by normal human skin fibroblasts were investigated with increasing passage. The increase of subculture number was associated with a constant increase in PG molecular size, which was particularly evident in cell layer extracts. In the cell layer, the ratio of DS-PGs/HS-PGs was markedly higher in early passage cultures. Moreover, the cell layer from young cells contained lower amounts of radioactivity incorporated into the most hydrophobic PG populations, suggesting that the PG core protein might also undergo significant modification with increasing subcultures. There was no significant difference in energy charge value between early and late passage cultures, whereas the NAD/NADH ratio was found to decrease markedly in senescent cells.

Original languageEnglish
Pages (from-to)263-269
Number of pages7
JournalCell Biochemistry and Function
Issue number4
Publication statusPublished - 1993



  • Human skin fibroblasts
  • Proteoglycans
  • Senescence

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

Cite this