Modifications of proteoglycans secreted into the growth medium by young and senescent human skin fibroblasts

Alberto Passi, Riccardo Albertini, Francesco Campagnari, Giancarlo De Luca

Research output: Contribution to journalArticle

Abstract

The properties of proteoglycans (PGs) secreted into the growth medium by normal young and senescent human skin fibroblasts (HFs) mere investigated. In both cases, the incorporation per cell of radioactive precursors into total PGs was similar. The polysaccharide chains of PGs from young and senescent HFs were mainly represented by galactosaminoglycuronans and showed a similar range of size distribution. However, galactosaminoglycuronans of PGs secreted by senescent HFs had a lower content of unsulphated disaccharides and a lower proportion of D-glucuronosyl residues. Moreover, senescent HFs released into the growth medium higher relative amounts of small PGs with chondroitin sulphate, dermatan sulphate chains, such as decorin.

Original languageEnglish
Pages (from-to)286-290
Number of pages5
JournalFEBS Letters
Volume402
Issue number2-3
DOIs
Publication statusPublished - Jan 27 1997

Keywords

  • Fibroblast
  • Glycosaminoglycan
  • Growth medium
  • Proteoglycan
  • Replicative senescence

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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