Modified fluidity and lipid composition in lipoproteins and platelet membranes from diabetic patients

R. A. Rabini, G. Ferretti, R. Galassi, M. Taus, G. Curatola, A. Tangorra, P. Fumelli, L. Mazzanti

Research output: Contribution to journalArticle

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Abstract

The interaction between lipoproteins and the platelet membrane has been proved to cause a modification in cellular functions. We studied 12 men with insulin-dependent diabetes mellitus (IDDM), 14 men with noninsulin-dependent diabetes mellitus (NIDDM), and 26 age-matched healthy men on the same diet. We determined fluidity by measuring the fluorescence polarization (P) of the probe 1,6-diphenyl-1,3,5-hexatriene (DPH) both in platelet membranes and in lipoproteins isolated by ultracentrifugation in NaBr density gradient. The lipid composition of lipoproteins and of platelet membranes was determined by enzymatic methods. The fluidity of platelet membranes was significantly increased both in patients affected by NIDDM and in subjects with IDDM compared with normal subjects. Low-density lipoproteins (LDL) showed an increased fluidity only in NIDDM patients. A percent increase in the triglyceride content was observed in all lipoprotein fractions in diabetic subjects. Increased phospholipid content was found in the platelet membranes from IDDM and NIDDM patients. The change in LDL fluidity observed in NIDDM patients might determine altered interactions between the lipoprotein and cellular receptors. The role of lipoproteins in the modulation of the platelet membrane properties in diabetes mellitus deserves further studies.

Original languageEnglish
Pages (from-to)381-385
Number of pages5
JournalClinical Biochemistry
Volume27
Issue number5
DOIs
Publication statusPublished - 1994

Fingerprint

Fluidity
Medical problems
Platelets
Lipoproteins
Blood Platelets
Type 2 Diabetes Mellitus
Membranes
Lipids
Chemical analysis
Type 1 Diabetes Mellitus
LDL Lipoproteins
Insulin
Diphenylhexatriene
Lipoprotein Receptors
Membrane Fluidity
Fluorescence Polarization
Ultracentrifugation
Phospholipids
Diabetes Mellitus
Triglycerides

Keywords

  • fluidity
  • insulin-dependent diabetes mellitus
  • lipid composition
  • lipoprotein
  • noninsulin-dependent diabetes mellitus
  • platelet membrane

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Modified fluidity and lipid composition in lipoproteins and platelet membranes from diabetic patients. / Rabini, R. A.; Ferretti, G.; Galassi, R.; Taus, M.; Curatola, G.; Tangorra, A.; Fumelli, P.; Mazzanti, L.

In: Clinical Biochemistry, Vol. 27, No. 5, 1994, p. 381-385.

Research output: Contribution to journalArticle

Rabini, RA, Ferretti, G, Galassi, R, Taus, M, Curatola, G, Tangorra, A, Fumelli, P & Mazzanti, L 1994, 'Modified fluidity and lipid composition in lipoproteins and platelet membranes from diabetic patients', Clinical Biochemistry, vol. 27, no. 5, pp. 381-385. https://doi.org/10.1016/0009-9120(94)90042-6
Rabini, R. A. ; Ferretti, G. ; Galassi, R. ; Taus, M. ; Curatola, G. ; Tangorra, A. ; Fumelli, P. ; Mazzanti, L. / Modified fluidity and lipid composition in lipoproteins and platelet membranes from diabetic patients. In: Clinical Biochemistry. 1994 ; Vol. 27, No. 5. pp. 381-385.
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