TY - JOUR
T1 - Modified salting-out method for DNA isolation from newborn cord blood nucleated cells
AU - Noguera, Nélida Inés
AU - Tallano, Carina Elizabet
AU - Bragós, Irma Margarita
AU - Milani, Angela Cristina
PY - 2000
Y1 - 2000
N2 - The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the β-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a considerable number of cord blood samples. By introducing a simple phenol/chloroform step, before ethanol precipitation of the nucleic acid, to certain samples, we were able to eliminate or substantially reduce the presence of inhibitors to PCR without having to re-extract the samples. (C) 2000 Wiley-Liss, Inc.
AB - The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the β-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a considerable number of cord blood samples. By introducing a simple phenol/chloroform step, before ethanol precipitation of the nucleic acid, to certain samples, we were able to eliminate or substantially reduce the presence of inhibitors to PCR without having to re-extract the samples. (C) 2000 Wiley-Liss, Inc.
KW - DNA extraction
KW - Nucleated cells
KW - PCR
KW - Phenol/chloroform
KW - Salting-out
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U2 - 10.1002/1098-2825(20001212)14:6<280::AID-JCLA6>3.0.CO;2-0
DO - 10.1002/1098-2825(20001212)14:6<280::AID-JCLA6>3.0.CO;2-0
M3 - Article
C2 - 11138610
AN - SCOPUS:0033672585
VL - 14
SP - 280
EP - 283
JO - Journal of Clinical Laboratory Analysis
JF - Journal of Clinical Laboratory Analysis
SN - 0887-8013
IS - 6
ER -