Modified salting-out method for DNA isolation from newborn cord blood nucleated cells

Nélida Inés Noguera, Carina Elizabet Tallano, Irma Margarita Bragós, Angela Cristina Milani

Research output: Contribution to journalArticle

Abstract

The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the β-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a considerable number of cord blood samples. By introducing a simple phenol/chloroform step, before ethanol precipitation of the nucleic acid, to certain samples, we were able to eliminate or substantially reduce the presence of inhibitors to PCR without having to re-extract the samples. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)280-283
Number of pages4
JournalJournal of Clinical Laboratory Analysis
Volume14
Issue number6
DOIs
Publication statusPublished - 2000

Keywords

  • DNA extraction
  • Nucleated cells
  • PCR
  • Phenol/chloroform
  • Salting-out

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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