Modified salting-out method for DNA isolation from newborn cord blood nucleated cells

Nélida Inés Noguera; Carina Elizabet Tallano; Irma Margarita Bragós; Angela Cristina Milani

doi:10.1002/1098-2825(20001212)14:6<280::AID-JCLA6>3.0.CO;2-0

Modified salting-out method for DNA isolation from newborn cord blood nucleated cells

Nélida Inés Noguera, Carina Elizabet Tallano, Irma Margarita Bragós, Angela Cristina Milani

Fondazione Santa Lucia

Research output: Contribution to journal › Article › peer-review

Abstract

The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the β-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a considerable number of cord blood samples. By introducing a simple phenol/chloroform step, before ethanol precipitation of the nucleic acid, to certain samples, we were able to eliminate or substantially reduce the presence of inhibitors to PCR without having to re-extract the samples. (C) 2000 Wiley-Liss, Inc.

Original language	English
Pages (from-to)	280-283
Number of pages	4
Journal	Journal of Clinical Laboratory Analysis
Volume	14
Issue number	6
DOIs	https://doi.org/10.1002/1098-2825(20001212)14:6<280::AID-JCLA6>3.0.CO;2-0
Publication status	Published - 2000

Keywords

DNA extraction
Nucleated cells
PCR
Phenol/chloroform
Salting-out

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1002/1098-2825(20001212)14:6<280::AID-JCLA6>3.0.CO;2-0

Cite this

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title = "Modified salting-out method for DNA isolation from newborn cord blood nucleated cells",

abstract = "The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the β-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a considerable number of cord blood samples. By introducing a simple phenol/chloroform step, before ethanol precipitation of the nucleic acid, to certain samples, we were able to eliminate or substantially reduce the presence of inhibitors to PCR without having to re-extract the samples. (C) 2000 Wiley-Liss, Inc.",

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T1 - Modified salting-out method for DNA isolation from newborn cord blood nucleated cells

AU - Noguera, Nélida Inés

AU - Tallano, Carina Elizabet

AU - Bragós, Irma Margarita

AU - Milani, Angela Cristina

PY - 2000

Y1 - 2000

N2 - The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the β-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a considerable number of cord blood samples. By introducing a simple phenol/chloroform step, before ethanol precipitation of the nucleic acid, to certain samples, we were able to eliminate or substantially reduce the presence of inhibitors to PCR without having to re-extract the samples. (C) 2000 Wiley-Liss, Inc.

AB - The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the β-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a considerable number of cord blood samples. By introducing a simple phenol/chloroform step, before ethanol precipitation of the nucleic acid, to certain samples, we were able to eliminate or substantially reduce the presence of inhibitors to PCR without having to re-extract the samples. (C) 2000 Wiley-Liss, Inc.

KW - DNA extraction

KW - Nucleated cells

KW - PCR

KW - Phenol/chloroform

KW - Salting-out

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Modified salting-out method for DNA isolation from newborn cord blood nucleated cells

Abstract

Keywords

ASJC Scopus subject areas

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