Abstract
The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the β-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a considerable number of cord blood samples. By introducing a simple phenol/chloroform step, before ethanol precipitation of the nucleic acid, to certain samples, we were able to eliminate or substantially reduce the presence of inhibitors to PCR without having to re-extract the samples. (C) 2000 Wiley-Liss, Inc.
| Original language | English |
|---|---|
| Pages (from-to) | 280-283 |
| Number of pages | 4 |
| Journal | Journal of Clinical Laboratory Analysis |
| Volume | 14 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2000 |
Keywords
- DNA extraction
- Nucleated cells
- PCR
- Phenol/chloroform
- Salting-out
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
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Cite this
Noguera, N. I., Tallano, C. E., Bragós, I. M., & Milani, A. C. (2000). Modified salting-out method for DNA isolation from newborn cord blood nucleated cells. Journal of Clinical Laboratory Analysis, 14(6), 280-283. https://doi.org/10.1002/1098-2825(20001212)14:6<280::AID-JCLA6>3.0.CO;2-0