Modulation of α-smooth muscle actin expression in fibroblasts by transforming growth factor-β isoforms: An in vivo and in vitro study

Guido Serini, Giulio Gabbiani

Research output: Contribution to journalArticlepeer-review

Abstract

Myofibroblasts are granulation tissue fibroblasts bearing ultrastructural and biochemical features of smooth muscle cells, such as cytoplasmic microfilaments and α-smooth muscle actin expression. They appear transiently during wound healing and more permanently during several pathologic situations such as fibrotic diseases. Transforming growth factor-β1 has been suggested to be an important promoter of the myofibroblastic phenotype. Here we show that (1) transforming growth factor-β2, like transforming growth factor-β1, induces myofibroblast formation in vivo and in vitro; (2) transforming growth factor-β3 acts as a negative regulator of the myofibroblastic phenotype in vivo but not in vitro; and (3) in vitro, the three different transforming growth factor-β isoforms are equally able to induce α-smooth muscle actin messenger RNA and protein expression in growing and quiescent cultured human and rat subcutaneous tissue fibroblasts. These data confirm that in vitro the behavior of the three different transforming growth factor-β isoforms is similar, whereas in vivo transforming growth factor-β isoforms possibly play different but complementary roles in myofibroblast modulation during wound repair.

Original languageEnglish
Pages (from-to)278-287
Number of pages10
JournalWound Repair and Regeneration
Volume4
Issue number2
Publication statusPublished - Apr 1996

ASJC Scopus subject areas

  • Dermatology
  • Surgery

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