Modulation of cell cycle-related protein expression by sodium butyrate in human non-small cell lung cancer cell lines

Cinzia Pellizzaro, Danila Coradini, Antonella Daniotti, Gabriella Abolafio, Maria Grazia Daidone

Research output: Contribution to journalArticlepeer-review

Abstract

To elucidate the mechanism of action of sodium butyrate (NaB), we examined its effect on the expression of some cell cycle-related proteins (cyclins D1 and E, p16ink4, p21wafl, p27kip1) in 2 human non-small cell lung cancer cell lines (NCI-460 and NCI-H23) characterized by wild- type and mutant TP53, respectively. The growth of both cell lines was inhibited in a dose-dependent manner and this process was accompanied by a modulation of cell cycle-related proteins. In NCI-H460, the p27kip1 and p16ink4 protein levels were markedly increased following Flag treatment, whereas p21waf1 was only slightly elevated, with a peak at 2 mM NaB, and p53 was unaffected by any concentration. By contrast, in NCI-123, a marked increase in p21waf1 protein was paralleled by decreased p53 levels, whereas all the other investigated proteins remained stable. The results suggest that NaB blocks the growth of both cell lines by induction of cyclin-dependent kinase inhibitors (in particular, p21waf1 in NCI-H23 and p27kip1 and p16ink4 in NCI-H460) through a p53-dependent or p53-independent mechanism, and open up interesting perspectives for the use of NaG as an alternative or additional strategy in the treatment of non-small cell lung carcinoma.

Original languageEnglish
Pages (from-to)654-657
Number of pages4
JournalInternational Journal of Cancer
Volume91
Issue number5
DOIs
Publication statusPublished - Mar 1 2001

Keywords

  • Cell cycle-related proteins
  • Lung cancer cell lines
  • Sodium butyrate

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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