Modulation of endothelial cell functions by different molecular species of interleukin 1

E. Dejana, F. Breviario, A. Erroi, F. Bussolino, L. Mussoni, M. Gramse, G. Pintucci, B. Casali, C. A. Dinarello, J. Van Damme

Research output: Contribution to journalArticlepeer-review

Abstract

Different molecular species of interleukin 1 (IL 1) were examined for the spectrum of responses elicited in human endothelial cells (HEC), including synthesis of prostacyclin (PGI2), tissue-type procoagulant activity (PCA), platelet activating factor (PAF), and plasminogen activator inhibitor (PA-I). The IL 1 preparations utilized for the present study included a natural, partially purified IL 1, a preparation purified to homogeneity with extensive homology with the derived aminoacid IL 1β (pI7) sequence denominated '22K factor', murine recombinant IL 1α, human recombinant IL 1α (PI5) and β (pI7). Natural, partially purified IL 1, a mixture of α and β species, induced the entire spectrum of responses in HEC. Production of PA-I was elicited by all forms of IL 1 tested. PGI2 and PCA were elicited by '22K factor' and by human recombinant IL 1β and α but not by murine recombinatnt IL 1α. PAF synthesis was stimulated by murine and human recombinant IL 1α but not by human recombinant IL 1β and 22K factor. Thus the available different molecular forms of IL 1 elicit largely but not completely overlapping patterns of responses in HEC. The IL 1 pathway of regulation of HEC functions might provide a basis for novel strategies in therapeutically oriented research on vessel wall disorders.

Original languageEnglish
Pages (from-to)695-699
Number of pages5
JournalBlood
Volume69
Issue number2
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Hematology

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