Modulation of Fas-Ligand (Fas-L) on human microglial cells: An in vitro study

S. Frigerio, V. Silei, E. Ciusani, G. Massa, G. M. Lauro, A. Salmaggi

Research output: Contribution to journalArticle

Abstract

The expression of Fas-Ligand (Fas-L) on microglia could be relevant in multiple sclerosis immunopathology. The present study was performed to evaluate in vitro the expression of Fas-L in human microglial cells both unstimulated and after stimulation with IFN-γ, β-IFN-1b and β-IFN-1b+IFN-γ. Cells were stimulated for 6,12, 24 and 48 h. Surface Fas-L was evaluated by flow cytometry, total Fas-L by Western blot, whereas mRNA for Fas-L was measured by RT-PCR. We also evaluated the capacity of microglial cells to induce, in vitro, apoptosis on Fas-positive T leukemia Jurkat cells. Our results showed a constitutive expression of Fas-L on microglia. IFN-γ downregulated the expression of the molecule, while β-IFN-1b and β-IFN-1b+IFN- γ did not. The amount of surface Fas-L was related to the ability of microglial cells to induce apoptosis in Fas-positive target cells, which was partly inhibited by blockade of the Fas-Fas-L pathway. Copyright (C) 2000.

Original languageEnglish
Pages (from-to)109-114
Number of pages6
JournalJournal of Neuroimmunology
Volume105
Issue number2
DOIs
Publication statusPublished - Jun 26 2000

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Keywords

  • Apoptosis
  • Fas-Ligand
  • Microglia

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

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