Modulation of fibronectin and thymic stromal cell-dependent thymocyte maturation by retinoic acid

Daniela Meco, Susanna Scarpa, Maddalena Napolitano, Marella Maroder, Diana Bellavia, Ruggero De Maria, Maria Ragano-Caracciolo, Luigi Frati, Andrea Modesti, Alberto Gulino, Isabella Screpanti

Research output: Contribution to journalArticle

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Abstract

Retinoic acid (RA) controls the differentiation of a variety of cell types, although its role in influencing T cell development and the mechanisms potentially involved have not been thoroughly investigated. To study the ability of RA to modulate T cell development, we established a thymic stromal cell line (TC-1S) that supports the phenotypic maturation of CD4-8- double negative (DN) or CD3-4-8- triple negative (TN) thymocyte precursors. Cocultures of either DN or TN thymocytes on a monolayer of TC-1S cells resulted in the appearance of thymocytes with a more mature phenotype (CD4+8+ double positive, CD4+ or CD8+ single positive, and CD3(low) cells). Double negative T cell contact with TC-1S cells also increased the production of fibronectin (FN) by the thymic stroma and the expression of the VLA-4 FN receptor on the DN cells. Ab-mediated inhibition of the interaction between FN and its receptors significantly reduced the level of induced T cell maturation. Addition of RA either to TC-1S cells alone or to the coculture with DN cells decreased stromal cell FN expression, antagonized DN cell-induced increase in stromal cell FN production and significantly inhibited in vitro thymocyte maturation. The effects of RA were likely mediated by RA acid receptors α and γ expressed both in DN thymocytes and TC-1S cells. Together these data suggest that FN/VLA-4 interaction may be an important component of stromal cell-dependent thymocyte phenotypic differentiation and that this interaction can be one of the targets for the influence of RA in T cell development.

Original languageEnglish
Pages (from-to)73-83
Number of pages11
JournalJournal of Immunology
Volume153
Issue number1
Publication statusPublished - Jul 1 1994

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Thymocytes
Stromal Cells
Tretinoin
Fibronectins
T-Lymphocytes
Integrin alpha4beta1
Coculture Techniques
Fibronectin Receptors
Integrin alpha5beta1
Phenotype
Cell Line
Acids

ASJC Scopus subject areas

  • Immunology

Cite this

Meco, D., Scarpa, S., Napolitano, M., Maroder, M., Bellavia, D., De Maria, R., ... Screpanti, I. (1994). Modulation of fibronectin and thymic stromal cell-dependent thymocyte maturation by retinoic acid. Journal of Immunology, 153(1), 73-83.

Modulation of fibronectin and thymic stromal cell-dependent thymocyte maturation by retinoic acid. / Meco, Daniela; Scarpa, Susanna; Napolitano, Maddalena; Maroder, Marella; Bellavia, Diana; De Maria, Ruggero; Ragano-Caracciolo, Maria; Frati, Luigi; Modesti, Andrea; Gulino, Alberto; Screpanti, Isabella.

In: Journal of Immunology, Vol. 153, No. 1, 01.07.1994, p. 73-83.

Research output: Contribution to journalArticle

Meco, D, Scarpa, S, Napolitano, M, Maroder, M, Bellavia, D, De Maria, R, Ragano-Caracciolo, M, Frati, L, Modesti, A, Gulino, A & Screpanti, I 1994, 'Modulation of fibronectin and thymic stromal cell-dependent thymocyte maturation by retinoic acid', Journal of Immunology, vol. 153, no. 1, pp. 73-83.
Meco D, Scarpa S, Napolitano M, Maroder M, Bellavia D, De Maria R et al. Modulation of fibronectin and thymic stromal cell-dependent thymocyte maturation by retinoic acid. Journal of Immunology. 1994 Jul 1;153(1):73-83.
Meco, Daniela ; Scarpa, Susanna ; Napolitano, Maddalena ; Maroder, Marella ; Bellavia, Diana ; De Maria, Ruggero ; Ragano-Caracciolo, Maria ; Frati, Luigi ; Modesti, Andrea ; Gulino, Alberto ; Screpanti, Isabella. / Modulation of fibronectin and thymic stromal cell-dependent thymocyte maturation by retinoic acid. In: Journal of Immunology. 1994 ; Vol. 153, No. 1. pp. 73-83.
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