Modulation of haemostatic function and prevention of experimental thrombosis by red wine in rats: A role for increased nitric oxide production

Tomasz Wollny, Luca Aiello, Donata Di Tommaso, Vincenzo Bellavia, Domenico Rotilio, Maria Benedetta Donati, Giovanni De Gaetano, Licia Iacoviello

Research output: Contribution to journalArticle

Abstract

1. The effects of ethyl alcohol and wine (red and white) on haemostatic parameters and experimental thrombosis were studied in rats; NO was evaluated as a possible mediator of these effects. 2. We found that red wine (12% alcohol) supplementation (8.4 ± 0.4 ml d-1 in drinking water, for 10 days) induced a marked prolongation of 'template' bleeding time (BT) (258 ± 13 vs 132 ± 13 s in controls; P <0.001), a decrease in platelet adhesion to fibrillar collagen (11.6 ± 1.0 vs 32.2 ± 1.3%; P <0.01) and a reduction in thrombus weight (1.45 ± 0.33 vs 3.27 ± 0.39 mg; P <0.01). 3. Alcohol-free red wine showed an effect similar to red wine. In contrast, neither ethyl alcohol (12%) nor white wine (12% alcohol) affected these systems. 4. All these effects were also observed after red wine i.v. injection (1 ml kg-1 of 1:4 dilution) 15 min before the experiments. 5. The effects of red wine were prevented by the NO inhibitor, N(ω)nitro-L-arginine-methyl ester (L-NAME). L-arginine, not D-arginine, reversed the effect of L-NAME on red wine infusion. 6. Red wine injection induced a 3 fold increase in total radical-trapping antioxidant parameter values of rat plasma with respect to controls, while white wine and alcohol did not show any effect. 7. Our study provides evidence that red wine modulates primary haemostasis and prevents experimental thrombosis in rats, independently of its alcohol content, by a NO-mediated mechanism.

Original languageEnglish
JournalBritish Journal of Pharmacology
Volume127
Issue number3
DOIs
Publication statusPublished - 1999

Keywords

  • Bleeding
  • Haemostasis
  • Nitric oxide
  • Plasma antioxidant capacity
  • Platelet adhesion
  • Venous thrombosis

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of 'Modulation of haemostatic function and prevention of experimental thrombosis by red wine in rats: A role for increased nitric oxide production'. Together they form a unique fingerprint.

Cite this