Abstract
Insulin-like growth factor 2 (IGF-2) is the major autocrine growth factor for neuroblastoma. IGF-2 mRNA can just be detected in SK-N-BE(2) cell line; higher levels are present in two clones derived from it [BE(2)-C; BE(2)-M17]. IGF-2 mRNA is increased by retinoic acid (RA) only in the clones. IGF-2 expression/induction is more marked in BE(2)-M17, which shows more RA-resistance (evaluated as growth inhibition, neurite outgrowth and induction of programmed cell death). Under RA exposure, the parental line shows a more pronounced growth inhibition, neurite outgrowth and programmed cell death, as compared to its clones. BE(2)-C cells also express type 1 IGF receptor mRNA, though with a different time course than for expression of IGF-2. The data suggest that IGF-2 expression is correlated with growth, and may counteract the growth retardation, neurite outgrowth and programmed cell death effects of retinoic acid. Therefore the autocrine pattern of IGF-2 production by neuroblastoma cells may promote RA-resistance.
| Original language | English |
|---|---|
| Pages (from-to) | 187-191 |
| Number of pages | 5 |
| Journal | Neuroscience Letters |
| Volume | 151 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Mar 19 1993 |
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Keywords
- Apoptosis
- Differentiation
- GHRH
- IGF-2
- Neuroblastoma
- Programmed cell death
- Retinoić acid
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Modulation of IGF-2 expression during growth and differentiation of human neuroblastoma cells : Retinoic acid may induce IGF-2. / Melino, Gerry; Stephanou, Anastasis; Annicchiarico-Petruzzelli, Margherita; Knight, Richard A.; Finazzi-Agro, Alessandro; Lightman, Stafford L.
In: Neuroscience Letters, Vol. 151, No. 2, 19.03.1993, p. 187-191.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Modulation of IGF-2 expression during growth and differentiation of human neuroblastoma cells
T2 - Retinoic acid may induce IGF-2
AU - Melino, Gerry
AU - Stephanou, Anastasis
AU - Annicchiarico-Petruzzelli, Margherita
AU - Knight, Richard A.
AU - Finazzi-Agro, Alessandro
AU - Lightman, Stafford L.
PY - 1993/3/19
Y1 - 1993/3/19
N2 - Insulin-like growth factor 2 (IGF-2) is the major autocrine growth factor for neuroblastoma. IGF-2 mRNA can just be detected in SK-N-BE(2) cell line; higher levels are present in two clones derived from it [BE(2)-C; BE(2)-M17]. IGF-2 mRNA is increased by retinoic acid (RA) only in the clones. IGF-2 expression/induction is more marked in BE(2)-M17, which shows more RA-resistance (evaluated as growth inhibition, neurite outgrowth and induction of programmed cell death). Under RA exposure, the parental line shows a more pronounced growth inhibition, neurite outgrowth and programmed cell death, as compared to its clones. BE(2)-C cells also express type 1 IGF receptor mRNA, though with a different time course than for expression of IGF-2. The data suggest that IGF-2 expression is correlated with growth, and may counteract the growth retardation, neurite outgrowth and programmed cell death effects of retinoic acid. Therefore the autocrine pattern of IGF-2 production by neuroblastoma cells may promote RA-resistance.
AB - Insulin-like growth factor 2 (IGF-2) is the major autocrine growth factor for neuroblastoma. IGF-2 mRNA can just be detected in SK-N-BE(2) cell line; higher levels are present in two clones derived from it [BE(2)-C; BE(2)-M17]. IGF-2 mRNA is increased by retinoic acid (RA) only in the clones. IGF-2 expression/induction is more marked in BE(2)-M17, which shows more RA-resistance (evaluated as growth inhibition, neurite outgrowth and induction of programmed cell death). Under RA exposure, the parental line shows a more pronounced growth inhibition, neurite outgrowth and programmed cell death, as compared to its clones. BE(2)-C cells also express type 1 IGF receptor mRNA, though with a different time course than for expression of IGF-2. The data suggest that IGF-2 expression is correlated with growth, and may counteract the growth retardation, neurite outgrowth and programmed cell death effects of retinoic acid. Therefore the autocrine pattern of IGF-2 production by neuroblastoma cells may promote RA-resistance.
KW - Apoptosis
KW - Differentiation
KW - GHRH
KW - IGF-2
KW - Neuroblastoma
KW - Programmed cell death
KW - Retinoić acid
UR - http://www.scopus.com/inward/record.url?scp=0027208906&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027208906&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(93)90017-F
DO - 10.1016/0304-3940(93)90017-F
M3 - Article
C2 - 8506078
AN - SCOPUS:0027208906
VL - 151
SP - 187
EP - 191
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 2
ER -