Modulation of MHC class II antigen expression in human myoblasts after treatment with IFN-γ

R. Mantegazza, S. M. Hughes, D. Mitchell, M. Travis, H. M. Blau, Lawrence Steinman

Research output: Contribution to journalArticlepeer-review

Abstract

Some investigators have proposed myoblast transfer as a potential therapy for the treatment of Duchenne muscular dystrophy. Little is known about the immunobiology of myoblast transplantation. Transplantation rejection is mediated to a large extent by CD8+ T cells, which recognize alloantigens encoded by class IHL A genes, and by CD4+ T cells, which recognize alloantigens encoded by class II HLA genes. Gamma interferon (IFN-γ) is a potent inducer of HLA class II molecules as well as β2-microglobulin, which is co-expressed with HLA class I. IFN-γ may be a critical cytokine involved in graft rejection. We purified human myoblasts by flow cytometry and incubated them in vitro for varying time periods with recombinant human IFN-γ. The inducibility of HLA-DR and -DP molecules raises a note of caution concerning possible rejection phenomenon which might occur following myoblast transplantation.

Original languageEnglish
Pages (from-to)1128-1132
Number of pages5
JournalNeurology
Volume41
Issue number7
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Arts and Humanities (miscellaneous)
  • Clinical Neurology
  • Neuroscience(all)

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