Modulation of paraoxonase 1 and 3 expression after moderate exercise training in the rat

Rita Romani, Gianna Evelina De Medio, Simona Di Tullio, Rosa Lapalombella, Irene Pirisinu, Vittoria Margonato, Arsenio Veicsteinas, Marina Marini, Gabriella Rosi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Paraoxonases (PONs) are a small family of anti-oxidant enzymes whose antiatherogenic activity is well known. The aim of the present study was the evaluation of the effects of moderate aerobic training on their expression using a rat model. In order to discriminate between PON1 and PON3 enzymatic activity, we took advantage of some differences in their substrate preferences. PON1 and PON3 enzymatic activities and their protein levels were analyzed in plasma and in liver microsomes, and their mRNA levels in the liver. Exercise training did not affect PON1 expression or enzymatic activity but increased PON3 mRNA, protein levels, and enzymatic activity. Training also induced variations in plasma membrane composition, including an increase in polyunsaturated and a decrease in mono- and di-unsaturated fatty acids. On the other hand, acute exercise inhibited PON activities while increasing PON3 protein content in liver microsomes and reversing the relative composition in mono-, di-, and poly-unsaturated fatty acids, suggesting that physical stress, by altering membrane composition, may impair PON release from liver membranes. In conclusion, we documented, for the first time, the presence of PON3 in rat serum and, notably, found that the upregulation of PON3, rather than PON1, appears to be associated with physical training.

Original languageEnglish
Pages (from-to)2036-2045
Number of pages10
JournalJournal of Lipid Research
Volume50
Issue number10
DOIs
Publication statusPublished - 2009

Fingerprint

Aryldialkylphosphatase
Liver
Rats
Modulation
Liver Microsomes
Exercise
Unsaturated Fatty Acids
Messenger RNA
Proteins
Membranes
Chemical analysis
Polyunsaturated fatty acids
Oxidants
Up-Regulation
Cell membranes
Cell Membrane
Enzymes
Serum
Plasmas
Substrates

Keywords

  • Arylesterase
  • Carboxylesterase
  • Lactonase
  • Membrane phospholipids
  • Mild exercise training
  • Protein expression
  • Real-time PCR

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology

Cite this

Romani, R., De Medio, G. E., Di Tullio, S., Lapalombella, R., Pirisinu, I., Margonato, V., ... Rosi, G. (2009). Modulation of paraoxonase 1 and 3 expression after moderate exercise training in the rat. Journal of Lipid Research, 50(10), 2036-2045. https://doi.org/10.1194/jlr.M800493-JLR200

Modulation of paraoxonase 1 and 3 expression after moderate exercise training in the rat. / Romani, Rita; De Medio, Gianna Evelina; Di Tullio, Simona; Lapalombella, Rosa; Pirisinu, Irene; Margonato, Vittoria; Veicsteinas, Arsenio; Marini, Marina; Rosi, Gabriella.

In: Journal of Lipid Research, Vol. 50, No. 10, 2009, p. 2036-2045.

Research output: Contribution to journalArticle

Romani, R, De Medio, GE, Di Tullio, S, Lapalombella, R, Pirisinu, I, Margonato, V, Veicsteinas, A, Marini, M & Rosi, G 2009, 'Modulation of paraoxonase 1 and 3 expression after moderate exercise training in the rat', Journal of Lipid Research, vol. 50, no. 10, pp. 2036-2045. https://doi.org/10.1194/jlr.M800493-JLR200
Romani R, De Medio GE, Di Tullio S, Lapalombella R, Pirisinu I, Margonato V et al. Modulation of paraoxonase 1 and 3 expression after moderate exercise training in the rat. Journal of Lipid Research. 2009;50(10):2036-2045. https://doi.org/10.1194/jlr.M800493-JLR200
Romani, Rita ; De Medio, Gianna Evelina ; Di Tullio, Simona ; Lapalombella, Rosa ; Pirisinu, Irene ; Margonato, Vittoria ; Veicsteinas, Arsenio ; Marini, Marina ; Rosi, Gabriella. / Modulation of paraoxonase 1 and 3 expression after moderate exercise training in the rat. In: Journal of Lipid Research. 2009 ; Vol. 50, No. 10. pp. 2036-2045.
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