Abstract
Neuroblastoma cell lines have been reported to contain two proopiomelanocortin (POMC) mRNA transcripts. We have now shown by immunocytochemistry and radioimmunoassay (RIA) that a number of neuroectodermally derived cell lines contain immunoreactive β-endorphin although cell concentrations were not characteristic of any tumour type. To explore further the functional significance of β-endorphin expression, we analysed neuroblastoma cell lines having intermediate (I), substrate adherent (S) and neuronal (N) phenotypes. No differences in cell β-endorphin content were detected. However, the expression of POMC mRNA and of immunoreactive β- endorphin was reduced within a few hours of treatment of these cell lines with retinoic acid. Culture of the cell lines in the presence of β-endorphin resulted in small but significant increases in growth. Although the POMC gene is in the same chromosomal segment as N-myc, which is normally amplified in neuroblastoma, no corresponding amplification of POMC could be demonstrated. The data suggest that POMC gene products may contribute to the autocrine/paracrine growth of neuroectodermal tumours.
| Original language | English |
|---|---|
| Pages (from-to) | 1402-1409 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 197 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1993 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Modulation of POMC expression in human neuroectodermal cells. / De Laurenzi, V.; Melino, G.; Knight, R. A.; Pierotti, A. R.; Cohen, P.
In: Biochemical and Biophysical Research Communications, Vol. 197, No. 3, 1993, p. 1402-1409.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Modulation of POMC expression in human neuroectodermal cells
AU - De Laurenzi, V.
AU - Melino, G.
AU - Knight, R. A.
AU - Pierotti, A. R.
AU - Cohen, P.
PY - 1993
Y1 - 1993
N2 - Neuroblastoma cell lines have been reported to contain two proopiomelanocortin (POMC) mRNA transcripts. We have now shown by immunocytochemistry and radioimmunoassay (RIA) that a number of neuroectodermally derived cell lines contain immunoreactive β-endorphin although cell concentrations were not characteristic of any tumour type. To explore further the functional significance of β-endorphin expression, we analysed neuroblastoma cell lines having intermediate (I), substrate adherent (S) and neuronal (N) phenotypes. No differences in cell β-endorphin content were detected. However, the expression of POMC mRNA and of immunoreactive β- endorphin was reduced within a few hours of treatment of these cell lines with retinoic acid. Culture of the cell lines in the presence of β-endorphin resulted in small but significant increases in growth. Although the POMC gene is in the same chromosomal segment as N-myc, which is normally amplified in neuroblastoma, no corresponding amplification of POMC could be demonstrated. The data suggest that POMC gene products may contribute to the autocrine/paracrine growth of neuroectodermal tumours.
AB - Neuroblastoma cell lines have been reported to contain two proopiomelanocortin (POMC) mRNA transcripts. We have now shown by immunocytochemistry and radioimmunoassay (RIA) that a number of neuroectodermally derived cell lines contain immunoreactive β-endorphin although cell concentrations were not characteristic of any tumour type. To explore further the functional significance of β-endorphin expression, we analysed neuroblastoma cell lines having intermediate (I), substrate adherent (S) and neuronal (N) phenotypes. No differences in cell β-endorphin content were detected. However, the expression of POMC mRNA and of immunoreactive β- endorphin was reduced within a few hours of treatment of these cell lines with retinoic acid. Culture of the cell lines in the presence of β-endorphin resulted in small but significant increases in growth. Although the POMC gene is in the same chromosomal segment as N-myc, which is normally amplified in neuroblastoma, no corresponding amplification of POMC could be demonstrated. The data suggest that POMC gene products may contribute to the autocrine/paracrine growth of neuroectodermal tumours.
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UR - http://www.scopus.com/inward/citedby.url?scp=0027749387&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1993.2633
DO - 10.1006/bbrc.1993.2633
M3 - Article
C2 - 8280157
AN - SCOPUS:0027749387
VL - 197
SP - 1402
EP - 1409
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -