Modulation of the human Harvey-ras oncogene expression by DNA methylation

M. G. Borrello, M. A. Pierotti, R. Donghi, I. Bongarzone, M. R. Cattadori, C. Traversari, P. Mondellini, G. Della Porta

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A clone of the human transforming Ha-ras oncogene (pT24-C3) was methylated 'in vitro' at its HpaII and HhaI sites and co-transfected with a selectable marker into NIH/3T3 cells. In the resulting colonies the normal or transformed morphology correlated respectively with a methylated or unmethylated status of the Ha-ras 1 promoter. A transfected, morphologically normal NIH/3T3 colony treated with 5'-azacytidine acquired a transformed phenotype growing in agar and in nude mice and showed demethylation of the promoter region of Ha-ras 1 gene. The cells of the reactivated colony produced human Ha-ras 1 mRNA and p21, and their DNA displayed transforming activity for NIH/3T3 recipient cells that was due to the original human Ha-ras 1 oncogene. We conclude that the methylation status of the promoter region of the human Ha-ras 1 modulates the expression and consequently the transforming activity of the oncogene.

Original languageEnglish
Pages (from-to)197-203
Number of pages7
JournalOncogene Research
Volume2
Issue number2
Publication statusPublished - 1988

Fingerprint

ras Genes
DNA Methylation
NIH 3T3 Cells
Genetic Promoter Regions
Azacitidine
Oncogenes
Nude Mice
Methylation
Agar
Clone Cells
Phenotype
Messenger RNA
DNA

ASJC Scopus subject areas

  • Cancer Research

Cite this

Borrello, M. G., Pierotti, M. A., Donghi, R., Bongarzone, I., Cattadori, M. R., Traversari, C., ... Della Porta, G. (1988). Modulation of the human Harvey-ras oncogene expression by DNA methylation. Oncogene Research, 2(2), 197-203.

Modulation of the human Harvey-ras oncogene expression by DNA methylation. / Borrello, M. G.; Pierotti, M. A.; Donghi, R.; Bongarzone, I.; Cattadori, M. R.; Traversari, C.; Mondellini, P.; Della Porta, G.

In: Oncogene Research, Vol. 2, No. 2, 1988, p. 197-203.

Research output: Contribution to journalArticle

Borrello, MG, Pierotti, MA, Donghi, R, Bongarzone, I, Cattadori, MR, Traversari, C, Mondellini, P & Della Porta, G 1988, 'Modulation of the human Harvey-ras oncogene expression by DNA methylation', Oncogene Research, vol. 2, no. 2, pp. 197-203.
Borrello MG, Pierotti MA, Donghi R, Bongarzone I, Cattadori MR, Traversari C et al. Modulation of the human Harvey-ras oncogene expression by DNA methylation. Oncogene Research. 1988;2(2):197-203.
Borrello, M. G. ; Pierotti, M. A. ; Donghi, R. ; Bongarzone, I. ; Cattadori, M. R. ; Traversari, C. ; Mondellini, P. ; Della Porta, G. / Modulation of the human Harvey-ras oncogene expression by DNA methylation. In: Oncogene Research. 1988 ; Vol. 2, No. 2. pp. 197-203.
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