Modulation of the locomotory capacity of human large granular lymphocytes

Nadia Polentarutti, Barbara Bottazzi, Claudia Balotta, Annalaura Erroi, Alberto Mantovani

Research output: Contribution to journalArticlepeer-review


The regulation of the migratory capacity of Percoll-purified large granular lymphocytes (LGL) into nitrocellulose filters was studied in a 2-hr assay with the use of modified Boyden chambers. Compounds that stimulate the natural killer cytotoxic function of LGL, such as interferons (natural β, recombinant αA, recombinant hybrid α A/D, recombinant γ), recombinant interleukin-2, and inactivated streptococci (OK 432), augmented the capacity of LGL to penetrate into filters spontaneously in the absence of chemoattractants in the lower compartment of the chamber. These compounds did not increase the LGL responsiveness to chemoattractants. Phorbol 12-myristate 13-acetate did not appreciably affect the locomotory capacity of LGL but augmented their cytotoxic activity. Thus the cytotoxic function and locomotion of LGL in response to biological response modifiers can be dissociated.

Original languageEnglish
Pages (from-to)204-212
Number of pages9
JournalCellular Immunology
Issue number1
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Cell Biology
  • Immunology


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