TY - JOUR
T1 - Modulation of the rod outer segment aerobic metabolism diminishes the production of radicals due to light absorption
AU - Calzia, Daniela
AU - Degan, Paolo
AU - Caicci, Federico
AU - Bruschi, Maurizio
AU - Manni, Lucia
AU - Ramenghi, Luca A.
AU - Candiano, Giovanni
AU - Traverso, Carlo Enrico
AU - Panfoli, Isabella
PY - 2018/3/1
Y1 - 2018/3/1
N2 - © 2018 Oxidative stress is a primary risk factor for both inflammatory and degenerative retinopathies. Our previous data on blue light-irradiated retinas demonstrated an oxidative stress higher in the rod outer segment (OS) than in the inner limb, leading to impairment of the rod OS extra-mitochondrial aerobic metabolism. Here the oxidative metabolism and Reactive Oxygen Intermediates (ROI) production was evaluated in purified bovine rod OS in function of exposure to different illumination conditions. A dose response was observed to varying light intensities and duration in terms of both ROI production and ATP synthesis. Pretreatment with resveratrol, inhibitor of F 1 F o -ATP synthase, or metformin, inhibitor of the respiratory complex I, significantly diminished the ROI production. Metformin also diminished the rod OS Complex I activity and reduced the maximal OS response to light in ATP production. Data show for the first time the relationship existing in the rod OS between its -aerobic- metabolism, light absorption, and ROI production. A beneficial effect was exerted by metformin and resveratrol, in modulating the ROI production in the illuminated rod OS, suggestive of their beneficial action also in vivo. Data shed new light on preventative interventions for cone loss secondary to rod damage due to oxidative stress.
AB - © 2018 Oxidative stress is a primary risk factor for both inflammatory and degenerative retinopathies. Our previous data on blue light-irradiated retinas demonstrated an oxidative stress higher in the rod outer segment (OS) than in the inner limb, leading to impairment of the rod OS extra-mitochondrial aerobic metabolism. Here the oxidative metabolism and Reactive Oxygen Intermediates (ROI) production was evaluated in purified bovine rod OS in function of exposure to different illumination conditions. A dose response was observed to varying light intensities and duration in terms of both ROI production and ATP synthesis. Pretreatment with resveratrol, inhibitor of F 1 F o -ATP synthase, or metformin, inhibitor of the respiratory complex I, significantly diminished the ROI production. Metformin also diminished the rod OS Complex I activity and reduced the maximal OS response to light in ATP production. Data show for the first time the relationship existing in the rod OS between its -aerobic- metabolism, light absorption, and ROI production. A beneficial effect was exerted by metformin and resveratrol, in modulating the ROI production in the illuminated rod OS, suggestive of their beneficial action also in vivo. Data shed new light on preventative interventions for cone loss secondary to rod damage due to oxidative stress.
KW - ATP synthase
KW - Diabetic dim light
KW - Light
KW - Metformin
KW - Oxidative stress
KW - Reactive oxygen intermediates
KW - Resveratrol
KW - Rod outer segment
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U2 - 10.1016/j.freeradbiomed.2018.01.029
DO - 10.1016/j.freeradbiomed.2018.01.029
M3 - Article
AN - SCOPUS:85041406706
VL - 117
SP - 110
EP - 118
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
SN - 0891-5849
ER -