Modulation of TNF production in a rat mast cell line (RBL) and monocytes (THP-1) by cAMP and glucocorticoids

A. Erroi, M. T. Demitri, M. Salmona, P. Ghezzi

Research output: Contribution to journalArticle

Abstract

Antigen-stimulated mast cells, like LPS-stimulated monocytes, produce tumor necrosis factor (TNF). We investigated whether TNF production was differentially regulated in the two cell types using two cell lines, a rat basophil leukemia (RBL) as a model for mast cells, and the monocytic leukemia cell line THP1. Agents that increased intracellular cAMP (forskolin, isobutylmethylxanthine (IBMX) and dibutyryl cAMP) and dexamethasone (DEX) inhibited TNF production in both cell types. An inhibitor of prostaglandin synthesis (indomethacin) and a nitric oxide (NO) donor, sodium nitroprusside (SNP) did not affect TNF production. Our results indicate that endogenous cAMP and glucocorticoids, but not prostaglandins or NO, are important regulators of TNF production in mast cells. TNF from mast cells and monocytes was equally in the susceptible of TNF to these inhibitors.

Original languageEnglish
Pages (from-to)17-22
Number of pages6
JournalInternational Journal of Immunopathology and Pharmacology
Volume11
Issue number1
Publication statusPublished - 1998

Keywords

  • CAMP
  • Mast cells
  • TNF

ASJC Scopus subject areas

  • Pharmacology

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