Modulation of Y-79 retinoblastoma cell differentiation and IRBP expression by dibutyryl cyclic AMP and laminin

G. Fassina, G. Paglialunga, D. M. Noonan, G. J. Chader, A. Albini

Research output: Contribution to journalArticle

Abstract

Y-79 retinoblastoma cells grown on a poly-D-lysine-coated substratum can assume a photoreceptor-like morphology and they express the retina-specific interphotoreceptor-retinoid-binding protein (IRBP) mRNA in abundance. In contrast, cells in suspension culture remain morphologically undifferentiated and exhibit a much lower IRBP message level. After treatment with the differentiating agent dibutyryl cyclic AMP (dbcAMP), monolayer cultures of Y-79 cells grown in serum-containing medium show a partial morphological and biochemical differentiation towards a Muller-glial-like phenotype. This is accompanied by a decrease in IRBP mRNA level with respect to that in Y-79 cells grown on poly-D-lysine. Attached cells which had been pretreated with laminin for three days do not demonstrate an altered IRBP mRNA level in response to dbcAMP as compared to cells not pretreated. Surprisingly, however, cells in suspension culture that were exposed to laminin and then to dbcAMP showed a 3-fold increase in IRBP mRNA level. Thus, expression of an important photoreceptor-specific marker is clearly modulated by both dbcAMP and laminin. Moreover, attachment itself may be a significant factor in determination of a neural or neuro-retinal cell phenotype.

Original languageEnglish
Pages (from-to)745-751
Number of pages7
JournalInternational Journal of Oncology
Volume2
Issue number5
Publication statusPublished - 1993

Fingerprint

Bucladesine
Retinoblastoma
Laminin
Cell Differentiation
Messenger RNA
Lysine
Suspensions
Phenotype
interstitial retinol-binding protein
Neuroglia
Retina
Serum

Keywords

  • cyclic AMP
  • differentiation
  • laminin
  • retinoblastoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Modulation of Y-79 retinoblastoma cell differentiation and IRBP expression by dibutyryl cyclic AMP and laminin. / Fassina, G.; Paglialunga, G.; Noonan, D. M.; Chader, G. J.; Albini, A.

In: International Journal of Oncology, Vol. 2, No. 5, 1993, p. 745-751.

Research output: Contribution to journalArticle

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AU - Paglialunga, G.

AU - Noonan, D. M.

AU - Chader, G. J.

AU - Albini, A.

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