Modulatory effects of NDP-MSH in the regenerating liver after partial hepatectomy in rats

Caterina Lonati, Andrea Carlin, Patrizia Leonardi, Franco Valenza, Silvano Bosari, Anna Catania, Stefano Gatti

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Melanocortins are endogenous peptides that exert protective actions on the host physiology. The broad modulatory effects of these molecules suggest that they might influence the mediator network induced during liver regeneration. The research aim was to determine if melanocortin treatment alters liver molecular changes induced by partial hepatectomy (PH). Rats under isoflurane anesthesia were subjected to standard 70% PH or sham surgery. Animals received a single i.v. injection of Nle4,DPhe7-α-melanocyte stimulating hormone (NDP-MSH) or saline 30 min before surgery. Sacrifice was performed at time intervals between 4 and 72 h. A preliminary screening based on TaqMan low-density array (TLDA) identified 71 transcripts altered by PH. Real-time PCR analysis revealed that NDP-MSH modulated the expression of a substantial proportion of these transcripts including several chemokines and their receptors. The critical signaling pathway interleukin-6 (IL-6)/signal transducer and activator of transcription (STAT)/suppressor of cytokine signaling (SOCS) was significantly enhanced by NDP-MSH. Further, peptide treatment considerably reduced the decline of IκBα protein caused by PH. Although the final organ regeneration was not substantially affected, NDP-MSH modulated expression of cell cycle mediators and exerted subtle influences on hepatocyte replication. Most of the changes brought about by NDP-MSH, a peptide approved for clinical use, should be salutary during liver regeneration.

Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalPeptides
Volume50
DOIs
Publication statusPublished - 2013

Fingerprint

Melanocyte-Stimulating Hormones
Hepatectomy
Liver
Rats
Melanocortins
Liver Regeneration
Surgery
Peptides
Critical Pathways
Chemokine Receptors
Isoflurane
Physiology
Transcription
Transducers
Real-Time Polymerase Chain Reaction
Regeneration
Hepatocytes
Interleukin-6
Cell Cycle
Screening

Keywords

  • 70% partial hepatectomy (PH)
  • Interleukin 6 (IL-6)
  • Liver regeneration
  • Melanocortin peptides
  • Suppressor of cytokine signaling (SOCS)

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Modulatory effects of NDP-MSH in the regenerating liver after partial hepatectomy in rats. / Lonati, Caterina; Carlin, Andrea; Leonardi, Patrizia; Valenza, Franco; Bosari, Silvano; Catania, Anna; Gatti, Stefano.

In: Peptides, Vol. 50, 2013, p. 145-152.

Research output: Contribution to journalArticle

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