Modulatory role of angiotensin-II in the secretion of atrial natriuretic factor in rabbits

Massimo Volpe, Paolo Pepino, Giuseppe Lembo, Silvana Pignalosa, Alessandro F. Mele, Speranza Rubattu, Gianluigi Condorelli, Elvio Covino, Bruno Trimarco

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This study was designed to investigate whether the increase in circulating atrial natriuretic factor (ANF) levels produced by angiotensin II (Ang II) is a consequence of the hemodynamic changes or whether it occurs also in the absence of pressor changes. For this purpose in anesthetized and awake rabbits we evaluated the effects of Ang II (0.1 μg/kg·min) alone or during the simultaneous infusion of sodium nitroprusside (NP) at a dose titrated to abolish the pressor effects. Systemic blood pressure increased from 76 ± 4 to 113 ± 5 mm Hg (P <0.001) during Ang II and from 76 ± 2 to 75 ± 3 mm Hg (P = NS) during Ang II plus NP. The α-adrenergic agonist phenylephrine, used as a control, raised blood pressure from 65 ± 2 to 101 ± 8 mm Hg (P <0.001), and its pressor effect was abolished by the concomitant infusion of NP (64 ± 2 to 61 ± 1 mm Hg; P = NS). The increase in plasma ANF levels produced by Ang II alone (from 36.5 ± 5 to 237 ± 57 pg/ml; P <0.001) was not different from that observed during Ang II plus NP (from 46 ± 10 to 207 ± 88 pg/ml; P <0.001). In contrast, the stimulatory effect on ANF release of phenylephrine (from 56.1 ± 9 to 202 ± 40 pg/ml; P <0.001) was completely abolished when its pressor effects were prevented by the combined infusion of NP (from 58.5 ± 15 to 42.3 ± 10 pg/ml; P = NS). These results show that the stimulatory effect of Ang II on ANF release can be clearly dissociated from its pressor effect, whereas the increase in plasma ANF levels caused by phenylephrine is strictly related to its hemodynamic effect. Therefore, Ang II is capable of modulating ANF secretion in a manner that is independent of its pressor actions. In addition, our results suggest that ANF release is not solely linked to myocyte stretch.

Original languageEnglish
Pages (from-to)2427-2431
Number of pages5
Issue number5
Publication statusPublished - May 1991

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism


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