Molecular alterations of Barrett's esophagus on microdissected endoscopic biopsies

Solange Romagnoli, Massimo Roncalli, Daniela Graziani, Barbara Cassani, Elena Roz, Luigi Bonavina, Alberto Peracchia, Silvano Bosari, Guido Coggi

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Alterations in proto-oncogenes and tumor suppressor genes play a role in the sequence from Barrett's metaplasia to esophageal adenocarcinoma. The present study aims to ascertain whether molecular abnormalities take place in Barrett's metaplasia and low-grade dysplasia and to correlate them with the histological features of the esophageal mucosa. Forty-one formalin-fixed, paraffin-embedded endoscopic esophageal biopsies were classified according to the type of metaplastic changes (noncolumnar fundic and cardial metaplasia; columnar metaplasia, with and without intestinal features). After microdissection samples were examined for loss of heterozygosity (LOH) using polymorphic markers on 5q (D5S82), corresponding to APC (adenomatous polyposis coli) gene, 13q (CA repeat in intron 2 position 14815 to 14998 of the retinoblastoma gene), 17p (D17S513) corresponding to p53 locus, and for p53 mutations. Molecular alterations including LOH, allelic imbalance, and microsatellite instability could be detected in all types of metaplastic changes and sporadically in the squamous epithelium adjacent to the metaplastic tissue. Molecular alterations involving microsatellites D5S82 and the CA repeat inside the retinoblastoma gene were more frequent in nonintestinal metaplasia whereas those involving the p53 locus took place in columnar intestinal metaplasia and in low-grade dysplasia. Clonal changes were demonstrated in different metaplastic areas in three patients. Genetic alterations comprising LOH and microsatellite instability characterize Barrett's mucosa and appear related to the type of metaplastic change. Some of them precede the development of intestinal metaplasia, suggesting that genetic alterations take place earlier than previously thought.

Original languageEnglish
Pages (from-to)241-247
Number of pages7
JournalLaboratory Investigation
Volume81
Issue number2
Publication statusPublished - 2001

Fingerprint

Barrett Esophagus
Metaplasia
Loss of Heterozygosity
Biopsy
Retinoblastoma Genes
Microsatellite Instability
Allelic Imbalance
APC Genes
Microdissection
Proto-Oncogenes
Tumor Suppressor Genes
Paraffin
Microsatellite Repeats
Introns
Formaldehyde
Mucous Membrane
Adenocarcinoma
Epithelium
Mutation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Molecular alterations of Barrett's esophagus on microdissected endoscopic biopsies. / Romagnoli, Solange; Roncalli, Massimo; Graziani, Daniela; Cassani, Barbara; Roz, Elena; Bonavina, Luigi; Peracchia, Alberto; Bosari, Silvano; Coggi, Guido.

In: Laboratory Investigation, Vol. 81, No. 2, 2001, p. 241-247.

Research output: Contribution to journalArticle

Romagnoli, Solange ; Roncalli, Massimo ; Graziani, Daniela ; Cassani, Barbara ; Roz, Elena ; Bonavina, Luigi ; Peracchia, Alberto ; Bosari, Silvano ; Coggi, Guido. / Molecular alterations of Barrett's esophagus on microdissected endoscopic biopsies. In: Laboratory Investigation. 2001 ; Vol. 81, No. 2. pp. 241-247.
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