Molecular analysis of decreased interleukin-12 production in persons infected with human immunodeficiency virus

Claire Chougnet, Thomas A. Wynn, Mario Clerici, Alan L. Landay, Harold A. Kessler, Janice Rusnak, Gregory P. Melcher, Alan Sher, Gene M. Shearer

Research output: Contribution to journalArticle

Abstract

Human immunodeficiency virus (HIV) disease is associated with loss of type 1 responses, including interleukin (IL)-12 production. The dramatic drop in p70 production seen at early stages of disease was found not to be associated with a similarly decreased p40 mRNA expression. p35 mRNA expression was more extensively reduced than p40 mRNA expression at these early stages. Monocytes infected in vitro with HIV displayed decreased p35 expression and p70 production, suggesting that such decreased IL-12 expression may contribute to reduced IL-12 production in HIV-positive patients' cells. In addition, treatment of cells with IL-10 increased IL-10 mRNA expression and decreased p40 expression in both HIV-positive and - negative cells, while neutralization of IL-10 increased p40 mRNA levels. These observations, together with the observed hyperproduction of IL-10 in HIV-positive patients, may explain the dysregulation of IL-12 production seen in HIV disease.

Original languageEnglish
Pages (from-to)46-53
Number of pages8
JournalJournal of Infectious Diseases
Volume174
Issue number1
Publication statusPublished - 1996

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ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Chougnet, C., Wynn, T. A., Clerici, M., Landay, A. L., Kessler, H. A., Rusnak, J., Melcher, G. P., Sher, A., & Shearer, G. M. (1996). Molecular analysis of decreased interleukin-12 production in persons infected with human immunodeficiency virus. Journal of Infectious Diseases, 174(1), 46-53.