Molecular analysis of Fanconi anemia and mismatch repair genes in patients with colorectal carcinoma

Maria Colombino, Antonio Avallone, Francesco Izzo, Fabiana Tatangelo, Mario Budroni, Antonio Cossu, Francesco Galimi, Pasquale Comella, Gerardo Botti, Maria Cristina Sini, Francesco Tanda, Giuseppe Palmieri

Research output: Contribution to journalArticle

Abstract

A cohort of 206 consecutively-collected patients with colorectal carcinoma (CRC) were screened for germline mutations in the principal DNA mismatch repair (MMR) genes, MLH1 and MSH2, and in the Fanconi anemia (FA) genes involved in homologous recombination DNA repair. Mutation analysis was performed by denaturing high-performance liquid chromatography (DHPLC) and automated sequencing. Available paraffin-embedded tumor tissues were evaluated for gene expression by immunohistochemistry. Genes of the FA pathway were found to participate in CRC pathogenesis, being silenced during disease progression and metastasis formation. Conversely, MLH1 and MSH2 genes seem to be inactivated at earlier stages of the disease. Finally, very few (about 5%) cases presented a simultaneous inactivation of the MMR and FA genes. Overall, our findings indicated that: i) mismatch DNA repair remains the main mechanism to be altered at both germline and somatic levels among CRC patients; ii) functional impairments of mismatch DNA repair and FA-related repair may represent two different pathogenetic alterations which are concurring in colorectal cancer progression.

Original languageEnglish
Pages (from-to)899-904
Number of pages6
JournalOncology Reports
Volume25
Issue number4
DOIs
Publication statusPublished - Apr 2011

Fingerprint

Fanconi Anemia
DNA Mismatch Repair
Colorectal Neoplasms
Genes
Recombinational DNA Repair
Germ-Line Mutation
Paraffin
Disease Progression
Immunohistochemistry
High Pressure Liquid Chromatography
Neoplasm Metastasis
Gene Expression
Mutation
Neoplasms

Keywords

  • Colorectal tumorigenesis
  • DNA repair
  • Immunohistochemistry
  • Mutation analysis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Molecular analysis of Fanconi anemia and mismatch repair genes in patients with colorectal carcinoma. / Colombino, Maria; Avallone, Antonio; Izzo, Francesco; Tatangelo, Fabiana; Budroni, Mario; Cossu, Antonio; Galimi, Francesco; Comella, Pasquale; Botti, Gerardo; Sini, Maria Cristina; Tanda, Francesco; Palmieri, Giuseppe.

In: Oncology Reports, Vol. 25, No. 4, 04.2011, p. 899-904.

Research output: Contribution to journalArticle

Colombino, M, Avallone, A, Izzo, F, Tatangelo, F, Budroni, M, Cossu, A, Galimi, F, Comella, P, Botti, G, Sini, MC, Tanda, F & Palmieri, G 2011, 'Molecular analysis of Fanconi anemia and mismatch repair genes in patients with colorectal carcinoma', Oncology Reports, vol. 25, no. 4, pp. 899-904. https://doi.org/10.3892/or.2011.1169
Colombino, Maria ; Avallone, Antonio ; Izzo, Francesco ; Tatangelo, Fabiana ; Budroni, Mario ; Cossu, Antonio ; Galimi, Francesco ; Comella, Pasquale ; Botti, Gerardo ; Sini, Maria Cristina ; Tanda, Francesco ; Palmieri, Giuseppe. / Molecular analysis of Fanconi anemia and mismatch repair genes in patients with colorectal carcinoma. In: Oncology Reports. 2011 ; Vol. 25, No. 4. pp. 899-904.
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