Molecular analysis of glomerular diseases in renal biopsies: initial results of a collaborative international study. The International Study Group for Molecular Study of Kidney Biopsies.

C. Esposito, L. J. Striker, A. Patel, E. Peten, Z. H. Liu, H. Sakai, G. E. Striker

Research output: Contribution to journalArticle

Abstract

An accurate assessment of which patient with glomerular disease will progress to end-stage renal failure would be an important addition to establishing prognosis and evaluating therapeutic strategies. We previously found the development of glomerular scarring in animal models was preceded by an increase in glomerular type IV collagen mRNAs and that the level of scarring predicted the rate of progression. The purpose of this study was to determine whether these findings apply to human glomerular diseases using microdissected glomeruli and assessment of mRNA by competitive PCR. After showing that the levels of type IV collagen mRNAs were elevated in sclerotic glomeruli isolated from nephrectomies, we undertook this preliminary cross-sectional study of type IV collagen subchain mRNAs in renal biopsies in two of the leading causes of glomerulosclerosis, diabetic nephropathy, and membranous glomerulopathy. We found that glomerular type IV collagen mRNA levels were altered in disease-specific ways. The relative levels of the individual alpha-chains of type IV collagen depended on the anatomic site of the glomerular lesions. The alpha 2 type IV/alpha 3 type IV collagen mRNA ratio was high in diabetes mellitus, but not in membranous glomerulopathy. These data, coupled with those obtained from experimental animals, suggest that a dysregulation of basement collagen synthesis underlies progressive glomerular scarring. If these conclusions are verified in prospective studies it will be feasible to assess the risk of developing progressive glomerulosclerosis in the individual patient and to quantitatively assess therapeutic responses in a timely manner.

Original languageEnglish
Pages (from-to)209-217
Number of pages9
JournalProceedings of the Association of American Physicians
Volume108
Issue number3
Publication statusPublished - May 1996

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Collagen Type IV
Kidney
Biopsy
Messenger RNA
Cicatrix
Membranous Glomerulonephritis
Collagen Type III
Diabetic Nephropathies
Nephrectomy
Chronic Kidney Failure
Diabetes Mellitus
Collagen
Animal Models
Cross-Sectional Studies
Prospective Studies
Polymerase Chain Reaction
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)

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Molecular analysis of glomerular diseases in renal biopsies : initial results of a collaborative international study. The International Study Group for Molecular Study of Kidney Biopsies. / Esposito, C.; Striker, L. J.; Patel, A.; Peten, E.; Liu, Z. H.; Sakai, H.; Striker, G. E.

In: Proceedings of the Association of American Physicians, Vol. 108, No. 3, 05.1996, p. 209-217.

Research output: Contribution to journalArticle

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