Molecular analysis of human γ/δ+ clones from thymus and peripheral blood

G. Casorati, G. De Libero, A. Lanzavecchia, N. Migone

Research output: Contribution to journalArticle

Abstract

We analyzed the Vγ and Vδ gene usage in TCR-γ/δ-bearing T cell clones isolated from human peripheral blood and postnatal thymus using V-specific mAbs and Southern and Northern analyses. In peripheral blood most of the γ/δ cells express the Vγ9-JP-Cγ1 chain paired with a δ chain bearing the Vδ2 gene product. This heterodimer is very rare in the postnatal thymus, where a different and less restricted pairing of Vγ9 and Vδ2 chains is found. These findings indicate that physical constraints cannot explain the overrepresentation of a particular Vγ9-JP/Vδ2 heterodimer in the peripheral blood, and we discuss alternative mechanisms that may account for this differential distribution. In addition, this analysis allowed us to map the specificity of the δTCS1 mAb to Vδ1-Jδ1 and to identify at least five different expressed Vδ genes.

Original languageEnglish
Pages (from-to)1521-1535
Number of pages15
JournalJournal of Experimental Medicine
Volume170
Issue number5
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Immunology

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    Casorati, G., De Libero, G., Lanzavecchia, A., & Migone, N. (1989). Molecular analysis of human γ/δ+ clones from thymus and peripheral blood. Journal of Experimental Medicine, 170(5), 1521-1535.