Molecular analysis of the bcr rearrangement in a case of Ph'-negative blastic crisis of Ph'-positive chronic myelogenous leukemia

C. Melani, L. Canepa, M. Sessarego, M. Miglino, A. M. Ferraris, G. F. Geatani

Research output: Contribution to journalArticlepeer-review

Abstract

We describe here a patient with Ph'-positive chronic myelogenous leukemia (CML) who developed a Ph'-negative blastic crisis. The blast DNA was analyzed on two different occasions, at the beginning of the blastic phase and at the end, shortly before the patient's death. Although cells from both samples had no Ph' chromosome marker (not even a masked one) we could detect a rearrangement of the bcr gene in the second DNA sample, using a '3'-bcr' probe. The same probe and a '5'-bcr' probe failed to detect any rearranged band in the first DNA sample. No rearrangement was identified at the c-myc and N-ras loci, while a slight c-myc amplification was evident in both DNA samples tested.

Original languageEnglish
Pages (from-to)32-37
Number of pages6
JournalEuropean Journal of Haematology
Volume42
Issue number1
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Hematology

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