Molecular and biological characterization of a replication competent human immunodeficiency type 2 (HIV-2) proviral clone

G. Franchini, K. A. Fargnoli, F. Giombini, L. Jagodzinski, A. De Rossi, M. Bosch, G. Biberfeld, E. M. Fenyo, J. Albert, R. C. Gallo, F. Wong-Staal

Research output: Contribution to journalArticle

Abstract

We obtained complete genomic clones of human immunodeficiency virus type 2 (HIV-2) from the DNA of the neoplastic human cell line HUT 78 freshly infected with a HIV-2 isolate, strain SBL6669. The recombinant phage DNA was transfected into the lymphocytes of CD4-positive HUT 78 cell line to test the replication competence of the proviral DNA. One genomic clone, designated HIV-2(SBL/ISY), yielded retroviral particles after a few weeks of culture of the transfected cells. The HIV-2(SBL/ISY) clone contained a complete provirus and cellular flanking sequence. We obtained the DNA sequence of the provirus and compared it with the published sequence of two other HIV-2 isolates. The degree of variability among HIV-2 isolates is comparable to that observed among African HIV-1 isolates sequenced to date. Immunologically, HIV-2(SBL/ISY) is similar to the parental virus (HIV-2(SBL6669)) but differs in the envelope transmembrane protein that is truncated (gp32-34) in the parental virus and not in HIV-2(SBL/ISY) (gp41). Both the parental and the cloned viruses are infectious and cytopathic for some human T-cell lines, induce syncytia, and infect a human macrophage cell line (U397) in vitro. The availability of a biologically active HIV-2 clone provides the means to study the role and interaction of HIV-2 genes in vitro as well as to assess the functional similarities among HIV-1 and HIV-2 genes. Since HIV-2(SBL/ISY) cloned virus infects fresh peripheral blood T cells from Rhesus macaques in vitro and infects the same animal in vivo, its use in animals may represent a model for functional study of viral genes in vivo as well as for development of experimental approaches to prevent and cure retroviral infection in humans.

Original languageEnglish
Pages (from-to)2433-2437
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number7
Publication statusPublished - 1989

ASJC Scopus subject areas

  • General
  • Genetics

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    Franchini, G., Fargnoli, K. A., Giombini, F., Jagodzinski, L., De Rossi, A., Bosch, M., Biberfeld, G., Fenyo, E. M., Albert, J., Gallo, R. C., & Wong-Staal, F. (1989). Molecular and biological characterization of a replication competent human immunodeficiency type 2 (HIV-2) proviral clone. Proceedings of the National Academy of Sciences of the United States of America, 86(7), 2433-2437.