Molecular Basis of Drug Resistance in Aurora Kinases

Fiona Girdler, Fabio Sessa, Simona Patercoli, Fabrizio Villa, Andrea Musacchio, Stephen Taylor

Research output: Contribution to journalArticlepeer-review

Abstract

Aurora kinases have emerged as potential targets in cancer therapy, and several drugs are currently undergoing preclinical and clinical validation. Whether clinical resistance to these drugs can arise is unclear. We exploited a hypermutagenic cancer cell line to select mutations conferring resistance to a well-studied Aurora inhibitor, ZM447439. All resistant clones contained dominant point mutations in Aurora B. Three mutations map to residues in the ATP-binding pocket that are distinct from the "gatekeeper" residue. The mutants retain wild-type catalytic activity and were resistant to all of the Aurora inhibitors tested. Our studies predict that drug-resistant Aurora B mutants are likely to arise during clinical treatment. Furthermore, because the plasticity of the ATP-binding pocket renders Aurora B insensitive to multiple inhibitors, our observations indicate that the drug-resistant Aurora B mutants should be exploited as novel drug targets.

Original languageEnglish
Pages (from-to)552-562
Number of pages11
JournalChemistry and Biology
Volume15
Issue number6
DOIs
Publication statusPublished - Jun 23 2008

Keywords

  • CHEMBIO
  • SIGNALING

ASJC Scopus subject areas

  • Organic Chemistry

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