Molecular biomonitoring of a population of nurses handling antineoplastic drugs

Tommaso Cornetta, Luca Padua, Antonella Testa, Elena Ievoli, Fabiola Festa, Giovanna Tranfo, Luigi Baccelliere, Renata Cozzi

Research output: Contribution to journalArticle

Abstract

Many antineoplastic drugs have been found to have carcinogenic, mutagenic and teratogenic activity and so hospital personnel handling these substances are potentially exposed to health risk. Understanding this risk derived from protracted occupational exposure has great relevance even if the workers normally adopt individual and environmental protective measures. To address this question we have studied the presence of DNA and chromosome damage in a population of nurses employed in Italian oncology units and in matched controls. We used the comet assay to evidence the presence of DNA strand breaks, due to both acute and chronic exposure, and the micronucleus (MN) test, which is a measure of clastogenic and aneugenic events. Furthermore, since the individual response to the exogenous insults may be genetically determined, we studied the possible influence of single nucleotide polymorphism in XRCC1 and XRCC3 DNA repair genes on induced genetic damage. We also considered the effects of confounding factors like smoking, age and gender. The results indicated that the exposed subjects had significantly high levels of genetic damage. Age and gender were associated with increased values in MN, both in control and in exposed groups; the smoking habit affects MN frequency in controls, but not in workers. Furthermore we found that exposed subjects bearing at least one XRCC1 variant allele (399Gln) show higher values of MN. The present data provide the evidence to show that occupational exposure to antineoplastic drugs, even if in safety controlled conditions, represents a serious health risk. Furthermore we have shown that the presence of XRCC1 genetic polymorphism could contribute to increase the genetic damage in susceptible individuals who are occupationally exposed to dangerous substances.

Original languageEnglish
Pages (from-to)75-82
Number of pages8
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume638
Issue number1-2
DOIs
Publication statusPublished - Feb 1 2008

Fingerprint

Environmental Monitoring
Antineoplastic Agents
Nurses
Occupational Exposure
Smoking
Population
Hospital Personnel
Micronucleus Tests
DNA Breaks
Comet Assay
Health
Genetic Polymorphisms
DNA Repair
DNA Damage
Habits
Single Nucleotide Polymorphism
Chromosomes
Alleles
Safety
Genes

Keywords

  • Comet assay
  • DNA repair
  • Hospital personnel
  • Micronuclei
  • Safety precautions

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Molecular Biology

Cite this

Molecular biomonitoring of a population of nurses handling antineoplastic drugs. / Cornetta, Tommaso; Padua, Luca; Testa, Antonella; Ievoli, Elena; Festa, Fabiola; Tranfo, Giovanna; Baccelliere, Luigi; Cozzi, Renata.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 638, No. 1-2, 01.02.2008, p. 75-82.

Research output: Contribution to journalArticle

Cornetta, Tommaso ; Padua, Luca ; Testa, Antonella ; Ievoli, Elena ; Festa, Fabiola ; Tranfo, Giovanna ; Baccelliere, Luigi ; Cozzi, Renata. / Molecular biomonitoring of a population of nurses handling antineoplastic drugs. In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2008 ; Vol. 638, No. 1-2. pp. 75-82.
@article{5e993fed1c9749bb814cfb0cfb279d2c,
title = "Molecular biomonitoring of a population of nurses handling antineoplastic drugs",
abstract = "Many antineoplastic drugs have been found to have carcinogenic, mutagenic and teratogenic activity and so hospital personnel handling these substances are potentially exposed to health risk. Understanding this risk derived from protracted occupational exposure has great relevance even if the workers normally adopt individual and environmental protective measures. To address this question we have studied the presence of DNA and chromosome damage in a population of nurses employed in Italian oncology units and in matched controls. We used the comet assay to evidence the presence of DNA strand breaks, due to both acute and chronic exposure, and the micronucleus (MN) test, which is a measure of clastogenic and aneugenic events. Furthermore, since the individual response to the exogenous insults may be genetically determined, we studied the possible influence of single nucleotide polymorphism in XRCC1 and XRCC3 DNA repair genes on induced genetic damage. We also considered the effects of confounding factors like smoking, age and gender. The results indicated that the exposed subjects had significantly high levels of genetic damage. Age and gender were associated with increased values in MN, both in control and in exposed groups; the smoking habit affects MN frequency in controls, but not in workers. Furthermore we found that exposed subjects bearing at least one XRCC1 variant allele (399Gln) show higher values of MN. The present data provide the evidence to show that occupational exposure to antineoplastic drugs, even if in safety controlled conditions, represents a serious health risk. Furthermore we have shown that the presence of XRCC1 genetic polymorphism could contribute to increase the genetic damage in susceptible individuals who are occupationally exposed to dangerous substances.",
keywords = "Comet assay, DNA repair, Hospital personnel, Micronuclei, Safety precautions",
author = "Tommaso Cornetta and Luca Padua and Antonella Testa and Elena Ievoli and Fabiola Festa and Giovanna Tranfo and Luigi Baccelliere and Renata Cozzi",
year = "2008",
month = "2",
day = "1",
doi = "10.1016/j.mrfmmm.2007.08.017",
language = "English",
volume = "638",
pages = "75--82",
journal = "Mutation Research",
issn = "0027-5107",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Molecular biomonitoring of a population of nurses handling antineoplastic drugs

AU - Cornetta, Tommaso

AU - Padua, Luca

AU - Testa, Antonella

AU - Ievoli, Elena

AU - Festa, Fabiola

AU - Tranfo, Giovanna

AU - Baccelliere, Luigi

AU - Cozzi, Renata

PY - 2008/2/1

Y1 - 2008/2/1

N2 - Many antineoplastic drugs have been found to have carcinogenic, mutagenic and teratogenic activity and so hospital personnel handling these substances are potentially exposed to health risk. Understanding this risk derived from protracted occupational exposure has great relevance even if the workers normally adopt individual and environmental protective measures. To address this question we have studied the presence of DNA and chromosome damage in a population of nurses employed in Italian oncology units and in matched controls. We used the comet assay to evidence the presence of DNA strand breaks, due to both acute and chronic exposure, and the micronucleus (MN) test, which is a measure of clastogenic and aneugenic events. Furthermore, since the individual response to the exogenous insults may be genetically determined, we studied the possible influence of single nucleotide polymorphism in XRCC1 and XRCC3 DNA repair genes on induced genetic damage. We also considered the effects of confounding factors like smoking, age and gender. The results indicated that the exposed subjects had significantly high levels of genetic damage. Age and gender were associated with increased values in MN, both in control and in exposed groups; the smoking habit affects MN frequency in controls, but not in workers. Furthermore we found that exposed subjects bearing at least one XRCC1 variant allele (399Gln) show higher values of MN. The present data provide the evidence to show that occupational exposure to antineoplastic drugs, even if in safety controlled conditions, represents a serious health risk. Furthermore we have shown that the presence of XRCC1 genetic polymorphism could contribute to increase the genetic damage in susceptible individuals who are occupationally exposed to dangerous substances.

AB - Many antineoplastic drugs have been found to have carcinogenic, mutagenic and teratogenic activity and so hospital personnel handling these substances are potentially exposed to health risk. Understanding this risk derived from protracted occupational exposure has great relevance even if the workers normally adopt individual and environmental protective measures. To address this question we have studied the presence of DNA and chromosome damage in a population of nurses employed in Italian oncology units and in matched controls. We used the comet assay to evidence the presence of DNA strand breaks, due to both acute and chronic exposure, and the micronucleus (MN) test, which is a measure of clastogenic and aneugenic events. Furthermore, since the individual response to the exogenous insults may be genetically determined, we studied the possible influence of single nucleotide polymorphism in XRCC1 and XRCC3 DNA repair genes on induced genetic damage. We also considered the effects of confounding factors like smoking, age and gender. The results indicated that the exposed subjects had significantly high levels of genetic damage. Age and gender were associated with increased values in MN, both in control and in exposed groups; the smoking habit affects MN frequency in controls, but not in workers. Furthermore we found that exposed subjects bearing at least one XRCC1 variant allele (399Gln) show higher values of MN. The present data provide the evidence to show that occupational exposure to antineoplastic drugs, even if in safety controlled conditions, represents a serious health risk. Furthermore we have shown that the presence of XRCC1 genetic polymorphism could contribute to increase the genetic damage in susceptible individuals who are occupationally exposed to dangerous substances.

KW - Comet assay

KW - DNA repair

KW - Hospital personnel

KW - Micronuclei

KW - Safety precautions

UR - http://www.scopus.com/inward/record.url?scp=38049093335&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38049093335&partnerID=8YFLogxK

U2 - 10.1016/j.mrfmmm.2007.08.017

DO - 10.1016/j.mrfmmm.2007.08.017

M3 - Article

C2 - 17928012

AN - SCOPUS:38049093335

VL - 638

SP - 75

EP - 82

JO - Mutation Research

JF - Mutation Research

SN - 0027-5107

IS - 1-2

ER -