Abstract
Staphylococcus aureus is the leading etiologic agent of orthopedic implant infections. Here a ribocluster of 27 S. aureus strains underwent further molecular characterization and subtyping by multilocus sequence typing (MLST) and spa-typing. This cluster had been detected by automated ribotyping (with the EcoRI restriction enzyme) of 200 S. aureus isolates from periprosthetic infections of patients who underwent revision at the Rizzoli Orthopaedic Institute. The ribocluster, consisting of agr type III strains, with a 74% co-occurrence of bone sialoprotein-binding (bbp) and collagen-binding (cna) genes, lacked mecA and IS256, and exhibited a high prevalence of the toxic shock syndrome toxin gene (tst, 85%). Strains' relatedness was analyzed by BURP and eBURST. Two predominant spa types, t012 (32%) and t021 (36%), and one predominant sequence type, ST30 (18/27, 67%) were identified: A S. aureus lineage spread worldwide belonging to MLST CC30. Two new sequence types (ST2954, ST2960) and one new spa type (t13129) were detected for the first time. Interestingly, the 27-strain cluster detected by ribotyping corresponded exactly to MLST CC30, the sole CC identified by eBURST.
| Original language | English |
|---|---|
| Article number | 8 |
| Journal | Frontiers in cellular and infection microbiology |
| Volume | 6 |
| Issue number | FEB |
| DOIs | |
| Publication status | Published - 2016 |
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Keywords
- Methicillin-sensitive Staphylococcus aureus
- Multilocus sequence typing
- Orthopedic implant infections
- Spa-typing
- Virulence factors
ASJC Scopus subject areas
- Microbiology
- Immunology
- Medicine(all)
- Microbiology (medical)
- Infectious Diseases
Cite this
Molecular characterization of a prevalent ribocluster of methicillin-sensitive Staphylococcus aureus from Orthopedic implant infections. Correspondence with MLST CC30. / Montanaro, Lucio; Ravaioli, Stefano; Ruppitsch, Werner; Campoccia, Davide; Pietrocola, G.; Visai, Livia; Speziale, Pietro; Allerberger, F.; Arciola, Carla Renata.
In: Frontiers in cellular and infection microbiology, Vol. 6, No. FEB, 8, 2016.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Molecular characterization of a prevalent ribocluster of methicillin-sensitive Staphylococcus aureus from Orthopedic implant infections. Correspondence with MLST CC30
AU - Montanaro, Lucio
AU - Ravaioli, Stefano
AU - Ruppitsch, Werner
AU - Campoccia, Davide
AU - Pietrocola, G.
AU - Visai, Livia
AU - Speziale, Pietro
AU - Allerberger, F.
AU - Arciola, Carla Renata
PY - 2016
Y1 - 2016
N2 - Staphylococcus aureus is the leading etiologic agent of orthopedic implant infections. Here a ribocluster of 27 S. aureus strains underwent further molecular characterization and subtyping by multilocus sequence typing (MLST) and spa-typing. This cluster had been detected by automated ribotyping (with the EcoRI restriction enzyme) of 200 S. aureus isolates from periprosthetic infections of patients who underwent revision at the Rizzoli Orthopaedic Institute. The ribocluster, consisting of agr type III strains, with a 74% co-occurrence of bone sialoprotein-binding (bbp) and collagen-binding (cna) genes, lacked mecA and IS256, and exhibited a high prevalence of the toxic shock syndrome toxin gene (tst, 85%). Strains' relatedness was analyzed by BURP and eBURST. Two predominant spa types, t012 (32%) and t021 (36%), and one predominant sequence type, ST30 (18/27, 67%) were identified: A S. aureus lineage spread worldwide belonging to MLST CC30. Two new sequence types (ST2954, ST2960) and one new spa type (t13129) were detected for the first time. Interestingly, the 27-strain cluster detected by ribotyping corresponded exactly to MLST CC30, the sole CC identified by eBURST.
AB - Staphylococcus aureus is the leading etiologic agent of orthopedic implant infections. Here a ribocluster of 27 S. aureus strains underwent further molecular characterization and subtyping by multilocus sequence typing (MLST) and spa-typing. This cluster had been detected by automated ribotyping (with the EcoRI restriction enzyme) of 200 S. aureus isolates from periprosthetic infections of patients who underwent revision at the Rizzoli Orthopaedic Institute. The ribocluster, consisting of agr type III strains, with a 74% co-occurrence of bone sialoprotein-binding (bbp) and collagen-binding (cna) genes, lacked mecA and IS256, and exhibited a high prevalence of the toxic shock syndrome toxin gene (tst, 85%). Strains' relatedness was analyzed by BURP and eBURST. Two predominant spa types, t012 (32%) and t021 (36%), and one predominant sequence type, ST30 (18/27, 67%) were identified: A S. aureus lineage spread worldwide belonging to MLST CC30. Two new sequence types (ST2954, ST2960) and one new spa type (t13129) were detected for the first time. Interestingly, the 27-strain cluster detected by ribotyping corresponded exactly to MLST CC30, the sole CC identified by eBURST.
KW - Methicillin-sensitive Staphylococcus aureus
KW - Multilocus sequence typing
KW - Orthopedic implant infections
KW - Spa-typing
KW - Virulence factors
UR - http://www.scopus.com/inward/record.url?scp=84991407458&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84991407458&partnerID=8YFLogxK
U2 - 10.3389/fcimb.2016.00008
DO - 10.3389/fcimb.2016.00008
M3 - Article
VL - 6
JO - Frontiers in cellular and infection microbiology
JF - Frontiers in cellular and infection microbiology
SN - 2235-2988
IS - FEB
M1 - 8
ER -