Molecular characterization of carbapenem resistant Acinetobacter baumannii and investigation of genetic diversity between local and international clones

Salman Shaheer Ahmed, Gokcen Dinc, Baldan Rossella, Emine Alp, Aysegul Ulu-Kilic, Willem J G Melchers, Joost Hopman, Andreas Voss

Research output: Contribution to journal › Article

2 Citations (Scopus)

Abstract

The emergence of Carbapenem-resistant Acinetobacter baumannii is a threat to modern medicine and has been recognised globally. Epidemic clones of A. baumannii are associated with hospital epidemics throughout the world causing serious ramifications. There are number of molecular typing techniques used for molecular characterisation, however, due to lack of resources molecular epidemiology remains unfledged in developing countries. In this study a total number of 205 carbapenem-resistant Acinetobacter species were collected and compared with European Clone I, II and III. PCR for and blaOXA-51-like was performed for confirmation and multiplex PCR was performed for blaOXA-23-like, blaOXA-24-like and blaOXA-58-like genes. Isolates harbouring carbapenemases were selected for molecular typing using PFGE, RAPD-PCR and REP-PCR and discriminatory index was performed. The cost of all molecular typing methods was calculated. The blaOXA-51-like gene was present in 188 (91.7%), and within these 188 isolates; blaOXA-23-like 36(19%), blaOXA-24-like 62(33%), 30(16%) carried blaOXA-58-like genes. PFGE typing revealed 13 clusters and 15 unique types, RAPD-PCR, 11 clusters and 23 unique types and REP-PCR, 8 clusters and no unique types. With 80% similarity cut-off value, 11 isolates were similar to EU clone I, 5 isolates were similar to EU II and 3 isolates were similar to EU III. Cost of molecular methods was compared between PFGE, RAPD-PCR and REP-PCR. PFGE was found to have high discriminatory power and RAPD-PCR was economical.

Original languageEnglish
Pages (from-to)1675-1682
Number of pages8
JournalJournal of Pure and Applied Microbiology
Volume10
Issue number3
Publication statusPublished - Sep 1 2016

Fingerprint

Random Amplified Polymorphic DNA Technique
Acinetobacter baumannii
Carbapenems
Molecular Typing
Clone Cells
Polymerase Chain Reaction
Genes
Costs and Cost Analysis
Modern 1601-history
Acinetobacter
Molecular Epidemiology
Multiplex Polymerase Chain Reaction
Developing Countries

Keywords

  • Carbapenem
  • Hospital infections
  • Molecular typing

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Molecular characterization of carbapenem resistant Acinetobacter baumannii and investigation of genetic diversity between local and international clones. / Ahmed, Salman Shaheer; Dinc, Gokcen; Rossella, Baldan; Alp, Emine; Ulu-Kilic, Aysegul; Melchers, Willem J G; Hopman, Joost; Voss, Andreas.

In: Journal of Pure and Applied Microbiology, Vol. 10, No. 3, 01.09.2016, p. 1675-1682.

Research output: Contribution to journal › Article

Ahmed, SS, Dinc, G, Rossella, B, Alp, E, Ulu-Kilic, A, Melchers, WJG, Hopman, J & Voss, A 2016, 'Molecular characterization of carbapenem resistant Acinetobacter baumannii and investigation of genetic diversity between local and international clones', Journal of Pure and Applied Microbiology, vol. 10, no. 3, pp. 1675-1682.
Ahmed SS, Dinc G, Rossella B, Alp E, Ulu-Kilic A, Melchers WJG et al. Molecular characterization of carbapenem resistant Acinetobacter baumannii and investigation of genetic diversity between local and international clones. Journal of Pure and Applied Microbiology. 2016 Sep 1;10(3):1675-1682.
Ahmed, Salman Shaheer ; Dinc, Gokcen ; Rossella, Baldan ; Alp, Emine ; Ulu-Kilic, Aysegul ; Melchers, Willem J G ; Hopman, Joost ; Voss, Andreas. / Molecular characterization of carbapenem resistant Acinetobacter baumannii and investigation of genetic diversity between local and international clones. In: Journal of Pure and Applied Microbiology. 2016 ; Vol. 10, No. 3. pp. 1675-1682.
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