Molecular characterization of commonly used cell lines for bone tumor research: A trans-European EuroBoNet effort

Laura Ottaviano, Karl Ludwig Schaefer, Melanie Gajewski, Wolfgang Huckenbeck, Stefan Baldus, Uwe Rogel, Carlos Mackintosh, Enrique De Alava, Ola Myklebost, Stine H. Kresse, Leonardo A. Meza-Zepeda, Massimo Serra, Anne Marie Cleton-Jansen, Pancras C W Hogendoorn, Horst Buerger, Thomas Aigner, Helmut E. Gabbert, Christopher Poremba

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Usage of cancer cell lines has repeatedly generated conflicting results provoked by differences among subclones or contamination with mycoplasm or other immortal mammalian cells. To overcome these limitations, we decided within the EuroBoNeT consortium to characterize a common set of cell lines including osteosarcomas (OS), Ewing sarcomas (ES), and chondrosarcomas (CS). DNA fingerprinting was used to guarantee the identity of all of the cell lines and to distinguish subclones of osteosarcoma cell line HOS. Screening for homozygous loss of 38 tumor suppressor genes by MLPA revealed deletion of CDKN2A as the most common event (15/36), strictly associated with absence of the CDKN2A (p16) protein. Ten cell lines showed missense mutations of the TP53 gene while another set of nine cell lines showed mutations resulting in truncation of the TP53 protein. Cells harboring missense mutations expressed high levels of nuclear TP53, while cell lines with nonsense mutations showed weak/absent staining for TP53. TP53wt cell lines usually expressed the protein in 2-10% of the cells. However, seven TP53wt osteosarcomas were negative for both mRNA and protein expression. Our analyses shed light on the correlation between immunohistochemical and genetic data for CDKN2A and TP53, and confirm the importance of these signaling pathways. The characterization of a substantial number of cell lines represents an important step to supply research groups with proven models for further advanced studies on tumor biology and may help to make results from different laboratories more comparable.

Original languageEnglish
Pages (from-to)40-51
Number of pages12
JournalGenes Chromosomes and Cancer
Volume49
Issue number1
DOIs
Publication statusPublished - Jan 2010

Fingerprint

Tumor Cell Line
Bone and Bones
Cell Line
Research
Osteosarcoma
Missense Mutation
Cyclin-Dependent Kinase Inhibitor p16
Tumor Suppressor Protein p53
Chondrosarcoma
Ewing's Sarcoma
DNA Fingerprinting
Nonsense Codon
p53 Genes
Tumor Suppressor Genes
Neoplasms
Proteins
Staining and Labeling
Messenger RNA
Mutation

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

Ottaviano, L., Schaefer, K. L., Gajewski, M., Huckenbeck, W., Baldus, S., Rogel, U., ... Poremba, C. (2010). Molecular characterization of commonly used cell lines for bone tumor research: A trans-European EuroBoNet effort. Genes Chromosomes and Cancer, 49(1), 40-51. https://doi.org/10.1002/gcc.20717

Molecular characterization of commonly used cell lines for bone tumor research : A trans-European EuroBoNet effort. / Ottaviano, Laura; Schaefer, Karl Ludwig; Gajewski, Melanie; Huckenbeck, Wolfgang; Baldus, Stefan; Rogel, Uwe; Mackintosh, Carlos; De Alava, Enrique; Myklebost, Ola; Kresse, Stine H.; Meza-Zepeda, Leonardo A.; Serra, Massimo; Cleton-Jansen, Anne Marie; Hogendoorn, Pancras C W; Buerger, Horst; Aigner, Thomas; Gabbert, Helmut E.; Poremba, Christopher.

In: Genes Chromosomes and Cancer, Vol. 49, No. 1, 01.2010, p. 40-51.

Research output: Contribution to journalArticle

Ottaviano, L, Schaefer, KL, Gajewski, M, Huckenbeck, W, Baldus, S, Rogel, U, Mackintosh, C, De Alava, E, Myklebost, O, Kresse, SH, Meza-Zepeda, LA, Serra, M, Cleton-Jansen, AM, Hogendoorn, PCW, Buerger, H, Aigner, T, Gabbert, HE & Poremba, C 2010, 'Molecular characterization of commonly used cell lines for bone tumor research: A trans-European EuroBoNet effort', Genes Chromosomes and Cancer, vol. 49, no. 1, pp. 40-51. https://doi.org/10.1002/gcc.20717
Ottaviano, Laura ; Schaefer, Karl Ludwig ; Gajewski, Melanie ; Huckenbeck, Wolfgang ; Baldus, Stefan ; Rogel, Uwe ; Mackintosh, Carlos ; De Alava, Enrique ; Myklebost, Ola ; Kresse, Stine H. ; Meza-Zepeda, Leonardo A. ; Serra, Massimo ; Cleton-Jansen, Anne Marie ; Hogendoorn, Pancras C W ; Buerger, Horst ; Aigner, Thomas ; Gabbert, Helmut E. ; Poremba, Christopher. / Molecular characterization of commonly used cell lines for bone tumor research : A trans-European EuroBoNet effort. In: Genes Chromosomes and Cancer. 2010 ; Vol. 49, No. 1. pp. 40-51.
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