Molecular characterization of Echinococcus granulosus in south-eastern Romania: Evidence of G1-G3 and G6-G10 complexes in humans

L. Piccoli, C. Bazzocchi, E. Brunetti, P. Mihailescu, C. Bandi, B. Mastalier, I. Cordos, M. Beuran, L. G. Popa, V. Meroni, F. Genco, C. Cretu

Research output: Contribution to journalArticle

Abstract

Clin Microbiol Infect Echinococcus granulosus is the aetiological agent of cystic echinococcosis (CE), which is a public health problem in many eastern European countries, particularly in Romania, where the infection causes a high number of human and animal cases. To shed light on the transmission patterns of the parasite, we performed a genotyping analysis on 60 cyst samples obtained from patients who live in south-eastern Romania and who underwent surgery for liver or lung CE. DNA was extracted from the endocysts or the cyst fluids, and fragments of cytochrome c oxidase subunit 1 and NADH dehydrogenase subunit 1 mitochondrial genes (cox1 and nd1, respectively) were amplified by PCR and sequenced. We found that most of the samples analysed (59/60) belonged to the G1-G3 complex (E. granulosus sensu stricto), which contains the most widespread and infective strains of the parasite. We also identified the first human patient infected by a non-G1-G3 genotype of E. granulosus in this country. As the DNA sequence of this cyst sample showed maximum homology with the G6-G10 complex (Echinococcus canadensis), this is, in all likelihood, a G7 genotype, which is often found in pigs and dogs in most countries of eastern and south-eastern Europe.

Original languageEnglish
Pages (from-to)578-582
Number of pages5
JournalClinical Microbiology and Infection
Volume19
Issue number6
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Echinococcus granulosus
Romania
Echinococcosis
Cysts
Parasites
Genotype
Echinococcus
Cyst Fluid
NADH Dehydrogenase
Eastern Europe
Mitochondrial Genes
Electron Transport Complex IV
Swine
Public Health
Dogs
Polymerase Chain Reaction
Lung
Liver
DNA
Infection

Keywords

  • Cystic echinococcosis
  • Echinococcus canadensis
  • Echinococcus granulosus
  • G1-G3 complex
  • G6-G10 complex
  • Genotype
  • Strain

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Molecular characterization of Echinococcus granulosus in south-eastern Romania : Evidence of G1-G3 and G6-G10 complexes in humans. / Piccoli, L.; Bazzocchi, C.; Brunetti, E.; Mihailescu, P.; Bandi, C.; Mastalier, B.; Cordos, I.; Beuran, M.; Popa, L. G.; Meroni, V.; Genco, F.; Cretu, C.

In: Clinical Microbiology and Infection, Vol. 19, No. 6, 06.2013, p. 578-582.

Research output: Contribution to journalArticle

Piccoli, L. ; Bazzocchi, C. ; Brunetti, E. ; Mihailescu, P. ; Bandi, C. ; Mastalier, B. ; Cordos, I. ; Beuran, M. ; Popa, L. G. ; Meroni, V. ; Genco, F. ; Cretu, C. / Molecular characterization of Echinococcus granulosus in south-eastern Romania : Evidence of G1-G3 and G6-G10 complexes in humans. In: Clinical Microbiology and Infection. 2013 ; Vol. 19, No. 6. pp. 578-582.
@article{6c444c0c577b491db5cab73f85e50cb3,
title = "Molecular characterization of Echinococcus granulosus in south-eastern Romania: Evidence of G1-G3 and G6-G10 complexes in humans",
abstract = "Clin Microbiol Infect Echinococcus granulosus is the aetiological agent of cystic echinococcosis (CE), which is a public health problem in many eastern European countries, particularly in Romania, where the infection causes a high number of human and animal cases. To shed light on the transmission patterns of the parasite, we performed a genotyping analysis on 60 cyst samples obtained from patients who live in south-eastern Romania and who underwent surgery for liver or lung CE. DNA was extracted from the endocysts or the cyst fluids, and fragments of cytochrome c oxidase subunit 1 and NADH dehydrogenase subunit 1 mitochondrial genes (cox1 and nd1, respectively) were amplified by PCR and sequenced. We found that most of the samples analysed (59/60) belonged to the G1-G3 complex (E. granulosus sensu stricto), which contains the most widespread and infective strains of the parasite. We also identified the first human patient infected by a non-G1-G3 genotype of E. granulosus in this country. As the DNA sequence of this cyst sample showed maximum homology with the G6-G10 complex (Echinococcus canadensis), this is, in all likelihood, a G7 genotype, which is often found in pigs and dogs in most countries of eastern and south-eastern Europe.",
keywords = "Cystic echinococcosis, Echinococcus canadensis, Echinococcus granulosus, G1-G3 complex, G6-G10 complex, Genotype, Strain",
author = "L. Piccoli and C. Bazzocchi and E. Brunetti and P. Mihailescu and C. Bandi and B. Mastalier and I. Cordos and M. Beuran and Popa, {L. G.} and V. Meroni and F. Genco and C. Cretu",
year = "2013",
month = "6",
doi = "10.1111/j.1469-0691.2012.03993.x",
language = "English",
volume = "19",
pages = "578--582",
journal = "Clinical Microbiology and Infection",
issn = "1198-743X",
publisher = "Elsevier B.V.",
number = "6",

}

TY - JOUR

T1 - Molecular characterization of Echinococcus granulosus in south-eastern Romania

T2 - Evidence of G1-G3 and G6-G10 complexes in humans

AU - Piccoli, L.

AU - Bazzocchi, C.

AU - Brunetti, E.

AU - Mihailescu, P.

AU - Bandi, C.

AU - Mastalier, B.

AU - Cordos, I.

AU - Beuran, M.

AU - Popa, L. G.

AU - Meroni, V.

AU - Genco, F.

AU - Cretu, C.

PY - 2013/6

Y1 - 2013/6

N2 - Clin Microbiol Infect Echinococcus granulosus is the aetiological agent of cystic echinococcosis (CE), which is a public health problem in many eastern European countries, particularly in Romania, where the infection causes a high number of human and animal cases. To shed light on the transmission patterns of the parasite, we performed a genotyping analysis on 60 cyst samples obtained from patients who live in south-eastern Romania and who underwent surgery for liver or lung CE. DNA was extracted from the endocysts or the cyst fluids, and fragments of cytochrome c oxidase subunit 1 and NADH dehydrogenase subunit 1 mitochondrial genes (cox1 and nd1, respectively) were amplified by PCR and sequenced. We found that most of the samples analysed (59/60) belonged to the G1-G3 complex (E. granulosus sensu stricto), which contains the most widespread and infective strains of the parasite. We also identified the first human patient infected by a non-G1-G3 genotype of E. granulosus in this country. As the DNA sequence of this cyst sample showed maximum homology with the G6-G10 complex (Echinococcus canadensis), this is, in all likelihood, a G7 genotype, which is often found in pigs and dogs in most countries of eastern and south-eastern Europe.

AB - Clin Microbiol Infect Echinococcus granulosus is the aetiological agent of cystic echinococcosis (CE), which is a public health problem in many eastern European countries, particularly in Romania, where the infection causes a high number of human and animal cases. To shed light on the transmission patterns of the parasite, we performed a genotyping analysis on 60 cyst samples obtained from patients who live in south-eastern Romania and who underwent surgery for liver or lung CE. DNA was extracted from the endocysts or the cyst fluids, and fragments of cytochrome c oxidase subunit 1 and NADH dehydrogenase subunit 1 mitochondrial genes (cox1 and nd1, respectively) were amplified by PCR and sequenced. We found that most of the samples analysed (59/60) belonged to the G1-G3 complex (E. granulosus sensu stricto), which contains the most widespread and infective strains of the parasite. We also identified the first human patient infected by a non-G1-G3 genotype of E. granulosus in this country. As the DNA sequence of this cyst sample showed maximum homology with the G6-G10 complex (Echinococcus canadensis), this is, in all likelihood, a G7 genotype, which is often found in pigs and dogs in most countries of eastern and south-eastern Europe.

KW - Cystic echinococcosis

KW - Echinococcus canadensis

KW - Echinococcus granulosus

KW - G1-G3 complex

KW - G6-G10 complex

KW - Genotype

KW - Strain

UR - http://www.scopus.com/inward/record.url?scp=84876787925&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876787925&partnerID=8YFLogxK

U2 - 10.1111/j.1469-0691.2012.03993.x

DO - 10.1111/j.1469-0691.2012.03993.x

M3 - Article

C2 - 22967309

AN - SCOPUS:84876787925

VL - 19

SP - 578

EP - 582

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

SN - 1198-743X

IS - 6

ER -