Abstract
Clin Microbiol Infect Echinococcus granulosus is the aetiological agent of cystic echinococcosis (CE), which is a public health problem in many eastern European countries, particularly in Romania, where the infection causes a high number of human and animal cases. To shed light on the transmission patterns of the parasite, we performed a genotyping analysis on 60 cyst samples obtained from patients who live in south-eastern Romania and who underwent surgery for liver or lung CE. DNA was extracted from the endocysts or the cyst fluids, and fragments of cytochrome c oxidase subunit 1 and NADH dehydrogenase subunit 1 mitochondrial genes (cox1 and nd1, respectively) were amplified by PCR and sequenced. We found that most of the samples analysed (59/60) belonged to the G1-G3 complex (E. granulosus sensu stricto), which contains the most widespread and infective strains of the parasite. We also identified the first human patient infected by a non-G1-G3 genotype of E. granulosus in this country. As the DNA sequence of this cyst sample showed maximum homology with the G6-G10 complex (Echinococcus canadensis), this is, in all likelihood, a G7 genotype, which is often found in pigs and dogs in most countries of eastern and south-eastern Europe.
| Original language | English |
|---|---|
| Pages (from-to) | 578-582 |
| Number of pages | 5 |
| Journal | Clinical Microbiology and Infection |
| Volume | 19 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2013 |
Fingerprint
Keywords
- Cystic echinococcosis
- Echinococcus canadensis
- Echinococcus granulosus
- G1-G3 complex
- G6-G10 complex
- Genotype
- Strain
ASJC Scopus subject areas
- Microbiology (medical)
- Infectious Diseases
Cite this
Molecular characterization of Echinococcus granulosus in south-eastern Romania : Evidence of G1-G3 and G6-G10 complexes in humans. / Piccoli, L.; Bazzocchi, C.; Brunetti, E.; Mihailescu, P.; Bandi, C.; Mastalier, B.; Cordos, I.; Beuran, M.; Popa, L. G.; Meroni, V.; Genco, F.; Cretu, C.
In: Clinical Microbiology and Infection, Vol. 19, No. 6, 06.2013, p. 578-582.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular characterization of Echinococcus granulosus in south-eastern Romania
T2 - Evidence of G1-G3 and G6-G10 complexes in humans
AU - Piccoli, L.
AU - Bazzocchi, C.
AU - Brunetti, E.
AU - Mihailescu, P.
AU - Bandi, C.
AU - Mastalier, B.
AU - Cordos, I.
AU - Beuran, M.
AU - Popa, L. G.
AU - Meroni, V.
AU - Genco, F.
AU - Cretu, C.
PY - 2013/6
Y1 - 2013/6
N2 - Clin Microbiol Infect Echinococcus granulosus is the aetiological agent of cystic echinococcosis (CE), which is a public health problem in many eastern European countries, particularly in Romania, where the infection causes a high number of human and animal cases. To shed light on the transmission patterns of the parasite, we performed a genotyping analysis on 60 cyst samples obtained from patients who live in south-eastern Romania and who underwent surgery for liver or lung CE. DNA was extracted from the endocysts or the cyst fluids, and fragments of cytochrome c oxidase subunit 1 and NADH dehydrogenase subunit 1 mitochondrial genes (cox1 and nd1, respectively) were amplified by PCR and sequenced. We found that most of the samples analysed (59/60) belonged to the G1-G3 complex (E. granulosus sensu stricto), which contains the most widespread and infective strains of the parasite. We also identified the first human patient infected by a non-G1-G3 genotype of E. granulosus in this country. As the DNA sequence of this cyst sample showed maximum homology with the G6-G10 complex (Echinococcus canadensis), this is, in all likelihood, a G7 genotype, which is often found in pigs and dogs in most countries of eastern and south-eastern Europe.
AB - Clin Microbiol Infect Echinococcus granulosus is the aetiological agent of cystic echinococcosis (CE), which is a public health problem in many eastern European countries, particularly in Romania, where the infection causes a high number of human and animal cases. To shed light on the transmission patterns of the parasite, we performed a genotyping analysis on 60 cyst samples obtained from patients who live in south-eastern Romania and who underwent surgery for liver or lung CE. DNA was extracted from the endocysts or the cyst fluids, and fragments of cytochrome c oxidase subunit 1 and NADH dehydrogenase subunit 1 mitochondrial genes (cox1 and nd1, respectively) were amplified by PCR and sequenced. We found that most of the samples analysed (59/60) belonged to the G1-G3 complex (E. granulosus sensu stricto), which contains the most widespread and infective strains of the parasite. We also identified the first human patient infected by a non-G1-G3 genotype of E. granulosus in this country. As the DNA sequence of this cyst sample showed maximum homology with the G6-G10 complex (Echinococcus canadensis), this is, in all likelihood, a G7 genotype, which is often found in pigs and dogs in most countries of eastern and south-eastern Europe.
KW - Cystic echinococcosis
KW - Echinococcus canadensis
KW - Echinococcus granulosus
KW - G1-G3 complex
KW - G6-G10 complex
KW - Genotype
KW - Strain
UR - http://www.scopus.com/inward/record.url?scp=84876787925&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876787925&partnerID=8YFLogxK
U2 - 10.1111/j.1469-0691.2012.03993.x
DO - 10.1111/j.1469-0691.2012.03993.x
M3 - Article
C2 - 22967309
AN - SCOPUS:84876787925
VL - 19
SP - 578
EP - 582
JO - Clinical Microbiology and Infection
JF - Clinical Microbiology and Infection
SN - 1198-743X
IS - 6
ER -